Number of the records: 1
Identification of Bifidobacterium strains from faeces of lambs
- 1.
SYSNO ASEP 0387185 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Identification of Bifidobacterium strains from faeces of lambs Author(s) Bunešová, V. (CZ)
Vlková, E. (CZ)
Killer, Jiří (UZFG-Y) RID, ORCID
Rada, V. (CZ)
Ročková, Š. (CZ)Source Title Small Ruminant Research. - : Elsevier - ISSN 0921-4488
Roč. 105, 1-3 (2012), 355-360Number of pages 6 s. Language eng - English Country NL - Netherlands Keywords bifidobacterium ; identification ; lambs Subject RIV GH - Livestock Nutrition R&D Projects GA523/08/1091 GA ČR - Czech Science Foundation (CSF) GD525/08/H060 GA ČR - Czech Science Foundation (CSF) Institutional support UZFG-Y - RVO:67985904 UT WOS 000305100900058 DOI 10.1016/j.smallrumres.2011.12.004 Annotation Bifidobacteria were enumerated and isolated from the faeces of lambs aged between 25 and 40 days using modified trypticase-phytone-yeast extract (TPY) agar supplemented with mupirocin and acetic acid. Nineteen strains were characterised and divided into 6 groups according their fermentation characteristics obtained by API 50 CHL and RAPID ID 32 tests and by randomly amplified polymorphic DNA (RAPD) profiles. One representative strain of each group was identified by sequencing by 16S rRNA and hsp60 genes sequencing as B. animalis ssp. animalis, B. choerinum, B. pseudolongum ssp. globosum, B. pseudocatenulatum. Examination of the DNA fingerprinting showed a genetic diversity between strains of B. animalis ssp. animalis and B. choerinum. Our result shows that some bifidobacterial species should not be host-specific or have a broad host spectrum. As bifidobacteria were determined as numerous bacterial group in the intestine of lambs on a milk diet (8.32 +/- 0.71 log CFU/g, mean count +/- S.D., n = 30), there is a reasonable presumption that bifidobacteria could be an effective probiotics for lambs. Identification techniques used in this study were found to be suitable for the classification of lamb origin bifidobacteria. Workplace Institute of Animal Physiology and Genetics Contact Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554 Year of Publishing 2013
Number of the records: 1