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Identification of Bifidobacterium strains from faeces of lambs

SYSNO ASEP	0387185
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Identification of Bifidobacterium strains from faeces of lambs
Author(s)	Bunešová, V. (CZ) Vlková, E. (CZ) Killer, Jiří (UZFG-Y)_{RID, ORCID} Rada, V. (CZ) Ročková, Š. (CZ)
Source Title	Small Ruminant Research. - : Elsevier - ISSN 0921-4488 Roč. 105, 1-3 (2012), 355-360
Number of pages	6 s.
Language	eng - English
Country	NL - Netherlands
Keywords	bifidobacterium ; identification ; lambs
Subject RIV	GH - Livestock Nutrition
R&D Projects	GA523/08/1091 GA ČR - Czech Science Foundation (CSF)
	GD525/08/H060 GA ČR - Czech Science Foundation (CSF)
Institutional support	UZFG-Y - RVO:67985904
UT WOS	000305100900058
DOI	10.1016/j.smallrumres.2011.12.004
Annotation	Bifidobacteria were enumerated and isolated from the faeces of lambs aged between 25 and 40 days using modified trypticase-phytone-yeast extract (TPY) agar supplemented with mupirocin and acetic acid. Nineteen strains were characterised and divided into 6 groups according their fermentation characteristics obtained by API 50 CHL and RAPID ID 32 tests and by randomly amplified polymorphic DNA (RAPD) profiles. One representative strain of each group was identified by sequencing by 16S rRNA and hsp60 genes sequencing as B. animalis ssp. animalis, B. choerinum, B. pseudolongum ssp. globosum, B. pseudocatenulatum. Examination of the DNA fingerprinting showed a genetic diversity between strains of B. animalis ssp. animalis and B. choerinum. Our result shows that some bifidobacterial species should not be host-specific or have a broad host spectrum. As bifidobacteria were determined as numerous bacterial group in the intestine of lambs on a milk diet (8.32 +/- 0.71 log CFU/g, mean count +/- S.D., n = 30), there is a reasonable presumption that bifidobacteria could be an effective probiotics for lambs. Identification techniques used in this study were found to be suitable for the classification of lamb origin bifidobacteria.
Workplace	Institute of Animal Physiology and Genetics
Contact	Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554
Year of Publishing	2013

Number of the records: 1