Number of the records: 1
Earliest stage of the tetrahedral nanochannel formation in cubosome particles from unilamellar nanovesicles
- 1.
SYSNO ASEP 0384663 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Earliest stage of the tetrahedral nanochannel formation in cubosome particles from unilamellar nanovesicles Author(s) Angelov, Borislav (UMCH-V) RID
Angelova, A. (FR)
Garamus, V. M. (DE)
Drechsler, M. (DE)
Willumeit, R. (DE)
Mutafchieva, R. (BG)
Štěpánek, Petr (UMCH-V) RID, ORCID
Lesieur, S. (FR)Source Title Langmuir. - : American Chemical Society - ISSN 0743-7463
Roč. 28, č. 48 (2012), s. 16647-16655Number of pages 9 s. Language eng - English Country US - United States Keywords cryoTEM electron microscopy ; SAXS ; soft matter Subject RIV CF - Physical ; Theoretical Chemistry R&D Projects GAP208/10/1600 GA ČR - Czech Science Foundation (CSF) Institutional support UMCH-V - RVO:61389013 CEZ AV0Z40500505 - UMCH-V (2005-2011) UT WOS 000311872900019 DOI 10.1021/la302721n Annotation Studies of nonequilibrium lipid polymorphism at the nanoscale contribute to the in-depth understanding of the structural pathways for formation of aqueous channels and emerging of channels-network ordering in liquid-crystalline (LC) nanovehicles. We present experimental structural evidence for the smallest tetrahedral-type lipid membrane aggregate, which involves completely formed nanochannels and occurs as an early intermediate state during the bilayer vesicle-to-cubosome particle transition. Nanovehicles are generated from a self-assembled lipid mixture and studied by means of high-resolution cryogenic transmission electron microscopy (cryo-TEM) and synchrotron radiation small-angle X-ray scattering (SAXS). The investigated lipid membrane composition allows for the stabilization of long-lived intermediates throughout the unilamellar vesicle-to-cubosome nanoparticle (NP) transformation at ambient temperature. The observed small cubosomic particles, with well-defined water channels, appear to be precursors of larger cubic membrane structures, thus confirming the theoretical modeling of nanochannel-network growth in diamond-type cubic lipid particles. The reported structural findings, highlighting that bilayer vesicle membrane packing and fusion are required for nanochanneled cubosome particle formation, are anticipated to advance the engineering of small lipid NPs with controllable channels for biomolecular loading and release. Workplace Institute of Macromolecular Chemistry Contact Eva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358 Year of Publishing 2013
Number of the records: 1