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Influence of the fetal bovine serum proteins on the growth of human osteoblast cells on graphene

SYSNO ASEP	0384165
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Influence of the fetal bovine serum proteins on the growth of human osteoblast cells on graphene
Author(s)	Kalbáčová, M. (CZ) Brož, A. (CZ) Kalbáč, Martin (UFCH-W)_{RID, ORCID}
Source Title	Journal of Biomedical Materials Research. Part A. - : Wiley - ISSN 1549-3296 100A, č. 11 (2012), s. 3001-3007
Number of pages	7 s.
Language	eng - English
Country	US - United States
Keywords	human osteoblast ; graphene ; fetal bovine serum
Subject RIV	CG - Electrochemistry
R&D Projects	IAA400400911 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR)
	KAN200100801 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR)
	GAP204/10/1677 GA ČR - Czech Science Foundation (CSF)
	GAP208/12/1062 GA ČR - Czech Science Foundation (CSF)
	ME09060 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Institutional support	UFCH-W - RVO:61388955
UT WOS	000309063400016
DOI	10.1002/jbm.a.34231
Annotation	The influence of single-layer graphene produced by chemical vapor deposition on human osteoblast cells under different conditions was studied. Measurements probed the ability of cells to adhere and proliferate on graphene compared with SiO2/Si substrates and standard tissue culture plastic when cells were incubated for the first 2 h in the presence or the absence of fetal bovine serum (FBS), thus influencing the initial, direct interaction of cells with the substrate. It was found that after 48 h of human osteoblast incubation on graphene films, there were a comparable number of cells of a similar size irrespective of the presence or the absence of serum proteins. On the other hand, a strong initial influence through the presence of FBS proteins on cell number and cell size was observed in the case of the SiO2/Si substrate and control plastic. Thus, our study showed that the initial presence/absence of FBS in the medium does not determine cell fate in the case of a graphene substrate, which is very unusual and different from the behavior of cells on other materials.
Workplace	J. Heyrovsky Institute of Physical Chemistry
Contact	Michaela Knapová, michaela.knapova@jh-inst.cas.cz, Tel.: 266 053 196
Year of Publishing	2013

Number of the records: 1