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Application of UV-Vis spectrophotometric and chemiluminescent methods for the evaluation of the antioxidant action of curcumin
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SYSNO ASEP 0383640 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Application of UV-Vis spectrophotometric and chemiluminescent methods for the evaluation of the antioxidant action of curcumin Author(s) Stanchev, Stancho (UOCHB-X) RID, ORCID
Pencheva, I. (BG)
Konstantinov, S. (BG)
Obreshkova, D. (BG)
Hadjimitova, V. (BG)Number of authors 5 Source Title Journal of the Serbian Chemical Society - ISSN 0352-5139
Roč. 77, č. 8 (2012), s. 1063-1069Number of pages 7 s. Language eng - English Country RS - Serbia Keywords curcumin ; antioxidant ; UV-Vis spectrophotometry ; DNA complexation ; chemiluminescence Subject RIV CC - Organic Chemistry CEZ AV0Z40550506 - UOCHB-X (2005-2011) UT WOS 000308830900008 DOI 10.2298/JSC110621032S Annotation Curcumin (1,7-bis(4-hydroxy-3-methoxyphenyl)-1,6-heptadiene-3,5-dione) is a natural biologically active substance with antioxidant activity. The ability of curcumin to inhibit free radical reactions can be used in the prevention of diseases such as cancer and coronary heart disease. UV-Vis spectrophotometric and chemiluminescent dynamic methods for determination of the antioxidant activity of curcumin were developed. The spectrophotometric method includes investigation of the interaction between DNA, isolated from HL-60 cells, and curcumin. The decreasing absorption of curcumin in the presence of HL-60 DNA against the blank sample can be a measurement for some complex formation between curcumin and DNA. The chemiluminescent method involves three tests for the detection of luminol- dependent chemiluminescence based on model systems that generate superoxide, hydroxide and hypochlorite radicals. The strongest decay of chemilunimescence was registered at the highest concentration of curcumin (100 mu mol L-1). Workplace Institute of Organic Chemistry and Biochemistry Contact asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Viktorie Chládková, Tel.: 232 002 434 Year of Publishing 2013
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