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Fluorescent Methods to Study Biological Membranes

SYSNO ASEP	0382433
Document Type	M - Monograph Chapter
R&D Document Type	Monograph Chapter
Title	Hydration and Mobility in Lipid Bilayers Probed by Time-Dependent Fluorescence Shift
Author(s)	Pokorná, Šárka (UFCH-W)_RID Olžyńska, Agnieszka (UFCH-W)_RID Jurkiewicz, Piotr (UFCH-W)_{RID, ORCID} Hof, Martin (UFCH-W)_{RID, ORCID}
Source Title	Fluorescent Methods to Study Biological Membranes. - Heidelberg : Springer, 2013 / Hof Martin - ISBN 978-3-642-33127-5
Pages	s. 141-159
Number of pages	19 s.
Number of pages	485
Publication form	Print - P
Language	eng - English
Country	DE - Germany
Keywords	Hofmeister series ; model lipid membranes ; oxidized lipids
Subject RIV	CF - Physical ; Theoretical Chemistry
R&D Projects	GAP208/10/1090 GA ČR - Czech Science Foundation (CSF)
	GEMEM/09/E006 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR)
Institutional support	UFCH-W - RVO:61388955
DOI	10.1007/4243_2012_46
Annotation	Biological membranes as an indispensable part of living organisms are permanently surrounded by the molecules of water. The presence of water is essential for maintaining their structure and functionality. Therefore, lipid bilayer hydration, mobility of the hydrated lipids, and their changes upon perturbations are appealing characteristics in the lipid membrane research. Time-dependent fluorescent shift (TDFS) measurements enable probing these properties in biologically relevant fully hydrated liquid crystalline lipid bilayers with a simple instrumentation and easy data treatment. Since the native lipid molecules do not fluoresce naturally, the extrinsic probing with a suitable fluorescent dye is necessary. There are a number of fluorescent membrane polarity probes designed for this purpose with different spectral properties and locations within the lipid bilayer. The basics of the technique are explained together with some useful additional considerations. The convenience of the TDFS method is demonstrated with examples from recent research on the study of the interactions of ions with lipid bilayers, and the monitoring of mobility and hydration changes along the bilayer normal upon addition of the oxidized phospholipids.
Workplace	J. Heyrovsky Institute of Physical Chemistry
Contact	Michaela Knapová, michaela.knapova@jh-inst.cas.cz, Tel.: 266 053 196
Year of Publishing	2014

Number of the records: 1