Preparation of stable Pd nanocubes and their use in biological labeling

Šlouf, Miroslav; Hrubý, Martin; Bakaeva, Zulfiya; Vlková, Helena; Nebesářová, Jana; Philimonenko, Anatoly; Hozák, Pavel

doi:https://dx.doi.org/10.1016/j.colsurfb.2012.05.023

Number of the records: 1

Preparation of stable Pd nanocubes and their use in biological labeling

1.

SYSNO ASEP	0381927
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Preparation of stable Pd nanocubes and their use in biological labeling
Author(s)	Šlouf, Miroslav (UMCH-V)_{RID, ORCID} Hrubý, Martin (UMCH-V)_{RID, ORCID} Bakaeva, Zulfiya (UMCH-V)_{RID, ORCID} Vlková, Helena (UMCH-V) Nebesářová, Jana (BC-A)_{RID, ORCID} Philimonenko, Anatoly (UMG-J) Hozák, Pavel (UMG-J)_{RID, ORCID}
Source Title	Colloids and Surfaces B-Biointerfaces. - : Elsevier - ISSN 0927-7765 Roč. 100, 1 December (2012), s. 205-208
Number of pages	4 s.
Language	eng - English
Country	NL - Netherlands
Keywords	nanoparticles ; palladium nanocubes ; electron microscopy
Subject RIV	EB - Genetics ; Molecular Biology
Subject RIV - cooperation	Institute of Molecular Genetics - Cell Biology
R&D Projects	KAN200520704 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR)
	GAP205/10/0348 GA ČR - Czech Science Foundation (CSF)
	GA202/09/2078 GA ČR - Czech Science Foundation (CSF)
	LC545 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Institutional support	UMCH-V - RVO:61389013
CEZ	AV0Z40500505 - UMCH-V (2005-2011)
	AV0Z50520514 - UMG-J (2005-2011)
	AV0Z60220518 - PAU-O, BC-A (2005-2011)
UT WOS	000307683900029
DOI	10.1016/j.colsurfb.2012.05.023
Annotation	Stable Pd nanocubes (PdNC) with the average size ~15 nm were prepared by the controlled reduction of sodium tetrachloropalladate with ascorbic acid in water, in the presence of polyvinylpyrrolidone and potassium bromide. Morphology of the particles was characterized by transmission electron microscopy (TEM) and their stability in the colloidal solution was verified by dynamic light scattering (DLS). It has been demonstrated that the Pd nanocubes can be distinguished from commercial Au nanospheres in a standard TEM microscope by means of automated image analysis. In the next step, the PdNC were successfully conjugated to immunoglobulin proteins and used for the detection of a specific protein (nucleophosmin) on ultrathin sections of HeLa cells. Our experiments showed that PdNC can be used for multiple immunolabeling in combination with commercial Au nanospheres.
Workplace	Institute of Macromolecular Chemistry
Contact	Eva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358
Year of Publishing	2013

Number of the records: 1