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Genetics of host response to Leishmania tropica in mice - different control of skin pathology, chemokine reaction, and iInvasion into spleen and liver
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SYSNO ASEP 0381628 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Genetics of host response to Leishmania tropica in mice - different control of skin pathology, chemokine reaction, and iInvasion into spleen and liver Author(s) Kobets, Tetyana (UMG-J) RID
Havelková, Helena (UMG-J)
Grekov, Igor (UMG-J) RID
Volkova, Valeriya (UMG-J)
Vojtíšková, Jarmila (UMG-J)
Slapničková, Martina (UMG-J)
Kurey, Irina (UMG-J)
Sohrabi, Yahya (UMG-J)
Svobodová, M. (CZ)
Demant, P. (US)
Lipoldová, Marie (UMG-J) RIDSource Title PLoS Neglected Tropical Diseases. - : Public Library of Science - ISSN 1935-2735
Roč. 6, č. 6 (2012), e1667Number of pages 12 s. Publication form Online - E Language eng - English Country US - United States Keywords leishmaniasis ; Leishmania tropica ; Leishmania major Subject RIV EB - Genetics ; Molecular Biology R&D Projects GA310/08/1697 GA ČR - Czech Science Foundation (CSF) GD310/08/H077 GA ČR - Czech Science Foundation (CSF) LC06009 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Institutional support UMG-J - RVO:68378050 UT WOS 000305945300011 DOI 10.1371/journal.pntd.0001667 Annotation Background: Leishmaniasis is a disease caused by protozoan parasites of genus Leishmania. The frequent involvement of Leishmania tropica in human leishmaniasis has been recognized only recently. Similarly as L. major, L. tropica causes cutaneous leishmaniasis in humans, but can also visceralize and cause systemic illness. The relationship between the host genotype and disease manifestations is poorly understood because there were no suitable animal models. Methods: We studied susceptibility to L. tropica, using BALB/c-c-STS/A (CcS/Dem) recombinant congenic (RC) strains, which differ greatly in susceptibility to L. major. Mice were infected with L. tropica and skin lesions, cytokine and chemokine levels in serum, and parasite numbers in organs were measured. Principal Findings: Females of BALB/c and several RC strains developed skin lesions. In some strains parasites visceralized and were detected in spleen and liver. Importantly, the strain distribution pattern of symptoms caused by L. tropica was different from that observed after L. major infection. Moreover, sex differently influenced infection with L. tropica and L. major. L. major-infected males exhibited either higher or similar skin pathology as females, whereas L. tropica-infected females were more susceptible than males. The majority of L. tropica-infected strains exhibited increased levels of chemokines CCL2, CCL3 and CCL5. CcS-16 females, which developed the largest lesions, exhibited a unique systemic chemokine reaction, characterized by additional transient early peaks of CCL3 and CCL5, which were not present in CcS-16 males nor in any other strain. Conclusion: Comparison of L. tropica and L. major infections indicates that the strain patterns of response are species-specific, with different sex effects and largely different host susceptibility genes. Workplace Institute of Molecular Genetics Contact Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Year of Publishing 2013
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