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Effect of heavy water on phospholipid membranes: experimental confirmation of molecular dynamics simulations

SYSNO ASEP	0381241
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Effect of heavy water on phospholipid membranes: experimental confirmation of molecular dynamics simulations
Author(s)	Beranová, Lenka (UFCH-W) Humpolíčková, Jana (UFCH-W)_RID Sýkora, Jan (UFCH-W)_RID Benda, Aleš (UFCH-W)_{RID, ORCID} Cwiklik, Lukasz (UFCH-W)_{RID, ORCID} Jurkiewicz, Piotr (UFCH-W)_{RID, ORCID} Gröbner, G. (SE) Hof, Martin (UFCH-W)_{RID, ORCID}
Source Title	Physical Chemistry Chemical Physics. - : Royal Society of Chemistry - ISSN 1463-9076 Roč. 14, č. 42 (2012), s. 14516-14522
Number of pages	7 s.
Language	eng - English
Country	GB - United Kingdom
Keywords	phospholipid membranes ; biophysics ; physical chemistry
Subject RIV	CF - Physical ; Theoretical Chemistry
R&D Projects	GEMEM/09/E006 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR)
	GBP208/12/G016 GA ČR - Czech Science Foundation (CSF)
Institutional support	UFCH-W - RVO:61388955
UT WOS	000309667100011
DOI	10.1039/C2CP41275F
Annotation	Although there were experimental indications that phospholipid bilayers hydrated with D2O express different biophysical properties compared with hydration by ordinary H2O, a molecular concept for this behavior difference was only recently proposed by a molecular dynamics simulations study [T. Róg et al., J. Phys. Chem. B, 2009, 113, 2378–2387]. Here we attempt to verify those theoretical predictions by fluorescence measurements on 1,2-dimyristoyl-sn-glycero-3-phosphocholine (DMPC) and 1-palmitoyl-2-oleoyl-sn-glycero-3-phosphocholine (POPC) membranes. Specifically, we determine the water isotope effect on headgroup hydration and mobility, lateral lipid diffusion and lipid backbone packing. Time-dependent fluorescence shift experiments show significantly slower dynamics and lower hydration of the headgroup region for a bilayer hydrated with D2O, an observation in good agreement with the calculated predicted differences in duration of lipid–lipid and lipid–water bridges and extent of water penetration into the bilayer, respectively. The water isotope effect on the lipid order parameter of the bilayer core (measured by fluorescence anisotropy) and lateral diffusion of lipid molecules (determined by two-focus fluorescence correlation spectroscopy) is close to the experimental errors of the experiments, however also refers to slightly more rigid organization of phospholipid bilayers in heavy water. This study confirms the view that the water isotope effect can be particularly found in time-resolved physicochemical properties of the membrane. Together with the simulations our experiments provide a comprehensive, molecular view on the effect of D2O on phospholipid bilayers.
Workplace	J. Heyrovsky Institute of Physical Chemistry
Contact	Michaela Knapová, michaela.knapova@jh-inst.cas.cz, Tel.: 266 053 196
Year of Publishing	2013

Number of the records: 1