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Native and denatured forms of proteins can be discriminated at edge plane carbon electrodes
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SYSNO ASEP 0380149 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Native and denatured forms of proteins can be discriminated at edge plane carbon electrodes Author(s) Ostatná, Veronika (BFU-R) RID, ORCID
Černocká, Hana (BFU-R) RID, ORCID
Kurzatkowska, K. (PL)
Paleček, Emil (BFU-R) RID, ORCIDNumber of authors 4 Source Title Analytica Chimica Acta. - : Elsevier - ISSN 0003-2670
Roč. 735, JUL (2012), s. 31-36Number of pages 6 s. Language eng - English Country NL - Netherlands Keywords protein denaturation ; carbon electrodes ; edge plane pyrolytic graphite Subject RIV BO - Biophysics R&D Projects KJB100040901 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) GAP301/11/2055 GA ČR - Czech Science Foundation (CSF) ME09038 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) CEZ AV0Z50040702 - BFU-R (2007-2013) UT WOS 000306150400004 DOI 10.1016/j.aca.2012.05.012 Annotation In an attempt to develop a label-free electrochemical method for detection of changes in protein structures based on oxidizability of tyrosine and tryptophan residues we tested different types of carbon electrodes. We found that using edge plane pyrolytic graphite electrode we can discriminate between native and denatured forms of human serum albumin and of other proteins. The electrochemical denaturation curve agreed reasonably well with that based on intrinsic fluorescence of tyrosine and tryptophan. It can be expected that the electrochemical method will be applicable to a large number of proteins and may become useful in biomedicine and proteomics. Workplace Institute of Biophysics Contact Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Year of Publishing 2013
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