Number of the records: 1

Metabolic activity and membrane integrity changes in Microcystis aeruginosa – new findings on hydrogen peroxide toxicity in cyanobacteria

SYSNO ASEP	0379236
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Metabolic activity and membrane integrity changes in Microcystis aeruginosa – new findings on hydrogen peroxide toxicity in cyanobacteria
Author(s)	Mikula, Přemysl (BU-J) Zezulka, Štěpán (BU-J)_ORCID Jančula, Daniel (BU-J)_RID Maršálek, Blahoslav (BU-J)_{ORCID, SAI}
Number of authors	4
Source Title	European Journal of Phycology. - : Taylor & Francis - ISSN 0967-0262 Roč. 47, č. 3 (2012), s. 195-206
Number of pages	12 s.
Language	eng - English
Country	GB - United Kingdom
Keywords	hydrogen peroxide ; cyanobacteria ; flow cytometry
Subject RIV	EF - Botanics
R&D Projects	FR-TI3/196 GA MPO - Ministry of Industry and Trade (MPO)
	TA01010356 GA TA ČR - Technology Agency of the Czech Republic (TA ČR)
Institutional support	BU-J - RVO:67985939
UT WOS	000308435700001
DOI	10.1080/09670262.2012.687144
Annotation	The aim of our study was to investigate the intracellular toxicity mechanisms of potentially anti-cyanobacterial agent hydrogen peroxide in Microcystis aeruginosa. Metabolic activity and cell membrane integrity were evaluated by flow cytometry in cyanobacteria exposed to the substance tested in the dark or light; the relationships between exposure effects and the kinetics of hydrogen peroxide decomposition were studied. Flow cytometry and chlorophyll a fluorescence measurements suggested that hydrogen peroxide exposure elicits an immediate decline of metabolic activity, and immediate changes of chlorophyll a fluorescence parameters, followed later by an increase in the percentage of membrane-compromised cells. When the concentration of hydrogen peroxide used was lethal, a significant drop in total cell counts was detected. Our study also confirmed that light is the critical factor affecting peroxide decomposition and thus greatly influences its toxicity.
Workplace	Institute of Botany
Contact	Martina Bartošová, martina.bartosova@ibot.cas.cz, ibot@ibot.cas.cz, Tel.: 271 015 242 ; Marie Jakšová, marie.jaksova@ibot.cas.cz, Tel.: 384 721 156-8
Year of Publishing	2013

Number of the records: 1