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Nitrogen source and growth stage of Candida albicans influence expression level of vacuolar aspartic protease Apr1p and carboxypeptidase Cpy1p
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SYSNO ASEP 0379073 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Nitrogen source and growth stage of Candida albicans influence expression level of vacuolar aspartic protease Apr1p and carboxypeptidase Cpy1p Author(s) Bauerová, Václava (UOCHB-X)
Pichová, Iva (UOCHB-X) RID, ORCID
Hrušková-Heidingsfeldová, Olga (UOCHB-X) RID, ORCIDNumber of authors 3 Source Title Canadian Journal of Microbiology - ISSN 0008-4166
Roč. 58, č. 5 (2012), s. 678-681Number of pages 4 s. Language eng - English Country CA - Canada Keywords Candida albicans ; carboxypeptidase Y ; nitrogen starvation ; vacuole Subject RIV CE - Biochemistry R&D Projects LC531 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) GA310/09/1945 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z40550506 - UOCHB-X (2005-2011) UT WOS 000303659500015 DOI 10.1139/W2012-030 Annotation The genes APR1 and CPY1 of the pathogenic yeast Candida albicans encode vacuolar aspartic protease and serine carboxypeptidase, respectively. Transcription of both APR1 and CPY1 was upregulated in mid-exponential phase, together with increasing size of the vacuoles, when C. albicans was cultivated in a rich medium at 30 °C. However, simultaneous up-regulation of protein synthesis occurred only for Cpy1p. Analysis of APR1 and CPY1 expression under nitrogen limited conditions revealed, that the genes were regulated on both the transcriptional and translational level and detectable amounts of Apr1p were synthesized only when C. albicans was grown in nitrogen limited media. Workplace Institute of Organic Chemistry and Biochemistry Contact asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Viktorie Chládková, Tel.: 232 002 434 Year of Publishing 2013
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