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Simple colorimetric methods for determination of sub-milligram amounts of ultra-high molecular weight polyethylene wear particles
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SYSNO ASEP 0377013 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Simple colorimetric methods for determination of sub-milligram amounts of ultra-high molecular weight polyethylene wear particles Author(s) Veselý, F. (CZ)
Zolotarevova, E. (CZ)
Špundová, M. (CZ)
Kaftan, Filip (UOCHB-X)
Šlouf, Miroslav (UMCH-V) RID, ORCID
Entlicher, G. (CZ)Number of authors 6 Source Title Acta Biomaterialia. - : Elsevier - ISSN 1742-7061
Roč. 8, č. 5 (2012), s. 1935-1938Number of pages 4 s. Language eng - English Country GB - United Kingdom Keywords joint replacement ; polyethylene wear particles ; wear particles determination ; colorimetric methods Subject RIV CC - Organic Chemistry R&D Projects 2B06096 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) NT12229 GA MZd - Ministry of Health (MZ) CEZ AV0Z40550506 - UOCHB-X (2005-2011) AV0Z40500505 - UMCH-V (2005-2011) UT WOS 000302989700028 DOI 10.1016/j.actbio.2012.01.010 Annotation New colorimetric methods are described for determination of sub-milligram amounts of ultra-high molecular weight polyethylene (UHMWPE) wear particles. These methods are based on the irreversible binding of the fluorescein-conjugated bovine serum albumin or the hydrophobic dye Oil Red O to wear particles. UHMWPE particles bind both substances from their solutions and thus decrease the absorbance of these solutions. The decrease is linearly dependent on the amount of added wear particles in the sub-milligram range suitable for practical use. The newly developed method offers improved accuracy and precision compared to Fourier transformed infrared spectroscopy (Slouf M. et al. Quantification of UHMWPE wear in periprosthetic tissues of hip arthoplasty: description of a new method based on IR and comparison with radiographic appearance. Wear 2008;265:674-684.) Workplace Institute of Organic Chemistry and Biochemistry Contact asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Viktorie Chládková, Tel.: 232 002 434 Year of Publishing 2013
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