Number of the records: 1

Differential response of Drosophila cell lines to extracellular adenosine

SYSNO ASEP	0376113
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Differential response of Drosophila cell lines to extracellular adenosine
Author(s)	Fleischmannová, J. (CZ) Kučerová, Lucie (BC-A)_{RID, ORCID} Šandová, Kateřina (BC-A) Steinbauerová, Veronika (BC-A) Brož, Václav (BC-A)_{RID, ORCID} Šimek, Petr (BC-A)_{RID, ORCID} Žurovec, Michal (BC-A)_{RID, ORCID}
Number of authors	7
Source Title	Insect Biochemistry and Molecular Biology. - : Elsevier - ISSN 0965-1748 Roč. 42, č. 5 (2012), s. 321-331
Number of pages	11 s.
Language	eng - English
Country	GB - United Kingdom
Keywords	adenosine recycling ; nucleoside transport ; Mbn2
Subject RIV	CE - Biochemistry
R&D Projects	LC06077 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
Institutional support	BC-A - RVO:60077344
CEZ	AV0Z50070508 - ENTU-I, BC-A (2005-2011)
UT WOS	000302515800002
DOI	10.1016/j.ibmb.2012.01.002
Annotation	Adenosine (Ado) is a crucial metabolite that influences proliferation of several vertebrate and invertebrate cells. Key proteins regulating Ado signaling, transport and metabolism are conserved among vertebrates and invertebrates. Here we show that Ado negatively influences viability, changes morphology and mitochondrial polarity of the Drosophila imaginal disc cell line (Cl.8ţ) via a mechanism exclusively dependent on cellular Ado uptake. High transport of Ado is followed by ATP production as a part of Ado salvation, which at higher concentrations may interfere with cellular homeostasis. In contrast, hematopoietic cell line Mbn2, which grows well in high Ado concentration, preferentially uses adenosine deaminase as a part of the purine catabolic pathway. Our results show that different types of Drosophila cell lines use different pathways for Ado conversion and suggest that such differences may be an important part of complex mechanisms maintaining energy homeostasis in the body.
Workplace	Biology Centre (since 2006)
Contact	Dana Hypšová, eje@eje.cz, Tel.: 387 775 214
Year of Publishing	2013
Electronic address	http://www.sciencedirect.com/science/article/pii/S0965174812000033

Number of the records: 1