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Human adipose tissue-derived mesenchymal stem cells expressing yeast cytosinedeaminase::uracil phosphoribosyltransferase inhibit intracerebral rat glioblastoma
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SYSNO ASEP 0376021 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Human adipose tissue-derived mesenchymal stem cells expressing yeast cytosinedeaminase::uracil phosphoribosyltransferase inhibit intracerebral rat glioblastoma Author(s) Altanerova, V. (SK)
Cihova, M. (SK)
Babič, Michal (UMCH-V) RID, ORCID
Rychly, B. (SK)
Ondicova, K. (SK)
Mravec, B. (SK)
Altaner, C. (SK)Source Title International Journal of Cancer. - : Wiley - ISSN 0020-7136
Roč. 130, č. 10 (2012), s. 2455-2463Number of pages 9 s. Language eng - English Country DE - Germany Keywords glioblastoma ; mesenchymal stem cells ; suicide gene therapy Subject RIV CD - Macromolecular Chemistry CEZ AV0Z40500505 - UMCH-V (2005-2011) UT WOS 000301579800025 DOI 10.1002/ijc.26278 Annotation Human adipose tissue-derived MSCs to express the suicide gene cytosine deaminase::uracil phosphoribosyltransferase to treat intracranial rat C6 glioblastoma was tested. Experiments simulated conditions of future clinical application for high-grade glioblastoma therapy by direct injections of therapeutic stem cells into tumor. Genetically modified therapeutic stem cells showed the tumor tropism when injected to a distant intracranial site and effectively inhibited glioblastoma growth after 5-fluorocytosine (5-FC) therapy. Coadministration of C6 cells and therapeutic stem cells with delayed 5-FC therapy improved the survival in a therapeutic stem cell dose-dependent manner and induced complete tumor regression. Continuous intracerebroventricular delivery of 5-FC using osmotic pump reduced the dose of prodrug required for the same therapeutic effect, and along with repeated administration of therapeutic stem cells increased the survival time without any detectable adverse effects. Workplace Institute of Macromolecular Chemistry Contact Eva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358 Year of Publishing 2013
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