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Screening ethnically diverse human embryonic stem cells identifies a chromosome 20 minimal amplicon conferring growth advantage
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SYSNO ASEP 0375448 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Screening ethnically diverse human embryonic stem cells identifies a chromosome 20 minimal amplicon conferring growth advantage Author(s) Amps, K. (GB)
Andrews, P.W. (GB)
Anyfantis, G. (GB)
Armstrong, L. (GB)
Avery, S. (SG)
Baharvand, H. (IR)
Baker, J. (US)
Baker, D. (GB)
Munoz, M. N. (GB)
Beil, S. (US)
Benvenisty, N. (IL)
Ben-Yosef, D. (IL)
Biancotti, J. C. (US)
Bosman, A. (CH)
Brena, R. M. (US)
Brison, D. (GB)
Caisander, G. (SE)
Camarasa, M. V. (GB)
Chen, J. M. (SG)
Chiao, E. (US)
Choi, Y. M. (KP)
Choo, E. (SG)
Collins, D. (GB)
Colman, A. (SG)
Crook, J. M. (SG)
Daley, G. Q. (US)
Dalton, A. (GB)
De Sousa, P. A. (GB)
Denning, C. (GB)
Downie, J. (GB)
Dvořák, P. (CZ)
Hampl, Aleš (UEM-P)Number of authors 125 Source Title Nature Biotechnology. - : Nature Publishing Group - ISSN 1087-0156
Roč. 29, č. 12 (2011), s. 1132-1144Number of pages 13 s. Language eng - English Country GB - United Kingdom Keywords comparative genomic hybridization ; copy number variation ; human es cells Subject RIV EB - Genetics ; Molecular Biology CEZ AV0Z50390703 - UEM-P (2007-2013) UT WOS 000298038700025 DOI 10.1038/nbt.2051 Annotation The International Stem Cell Initiative analyzed 125 human embryonic stem (ES) cell lines and 11 induced pluripotent stem (iPS) cell lines, from 38 laboratories worldwide, for genetic changes occurring during culture. Most lines were analyzed at an early and late passage. Single-nucleotide polymorphism (SNP) analysis revealed that they included representatives of most major ethnic groups. Most lines remained karyotypically normal, but there was a progressive tendency to acquire changes on prolonged culture, commonly affecting chromosomes 1, 12, 17 and 20. DNA methylation patterns changed haphazardly with no link to time in culture. Structural variants, determined from the SNP arrays, also appeared sporadically. No common variants related to culture were observed on chromosomes 1, 12 and 17, but a minimal amplicon in chromosome 20q11.21, including three genes expressed in human ES cells, ID1, BCL2L1 and HM13, occurred in >20% of the lines. Of these genes, BCL2L1 is a strong candidate for driving culture adaptation of ES cells. Workplace Institute of Experimental Medicine Contact Lenka Koželská, lenka.kozelska@iem.cas.cz, Tel.: 241 062 218, 296 442 218 Year of Publishing 2012
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