Number of the records: 1
The Arabidopsis YUCCA1 Flavin Monooxygenase Functions in the Indole-3-Pyruvic Acid Branch of Auxin Biosynthesis
- 1.
SYSNO ASEP 0374857 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title The Arabidopsis YUCCA1 Flavin Monooxygenase Functions in the Indole-3-Pyruvic Acid Branch of Auxin Biosynthesis Author(s) Stepanova, A.N. (US)
Yun, J. (US)
Robles, L.M. (US)
Novák, Ondřej (UEB-Q) RID, ORCID, SAI
He, W. (CN)
Guo, H.W. (CN)
Ljung, K. (SE)
Alonso, J.M. (US)Number of authors 8 Source Title Plant Cell. - : Oxford University Press - ISSN 1040-4651
Roč. 23, č. 11 (2011), s. 3961-3973Number of pages 14 s. Language eng - English Country US - United States Keywords PLANT DEVELOPMENT ; GLUCOSINOLATE BIOSYNTHESIS ; REPRODUCTIVE DEVELOPMENT ; MASS-SPECTROMETRY ; ALDEHYDE OXIDASE ; THALIANA ; GENE ; METABOLISM ; MUTANTS ; PATHWAY Subject RIV EF - Botanics R&D Projects GA301/08/1649 GA ČR - Czech Science Foundation (CSF) UT WOS 000298674200011 DOI 10.1105/tpc.111.088047 Annotation The effects of auxins on plant growth and development have been known for more than 100 years, yet our understanding of how plants synthesize this essential plant hormone is still fragmentary at best. Gene loss-and gain-of-function studies have conclusively implicated three gene families, CYTOCHROME P450 79B2/B3 (CYP79B2/B3), YUCCA (YUC), and TRYPTOPHAN AMINOTRANSFERASE OF ARABIDOPSIS1/TRYPTOPHAN AMINOTRANSFERASE-RELATED (TAA1/TAR), in the production of this hormone in the reference plant Arabidopsis thaliana. Each of these three gene families is believed to represent independent routes of auxin biosynthesis. Using a combination of pharmacological, genetic, and biochemical approaches, we examined the possible relationships between the auxin biosynthetic pathways defined by these three gene families. Our findings clearly indicate that TAA1/TARs and YUCs function in a common linear biosynthetic pathway that is genetically distinct from the CYP79B2/B3 route. In the redefined TAA1-YUC auxin biosynthetic pathway, TAA1/TARs are required for the production of indole-3-pyruvic acid (IPyA) from Trp, whereas YUCs are likely to function downstream. These results, together with the extensive genetic analysis of four pyruvate decarboxylases, the putative downstream components of the TAA1 pathway, strongly suggest that the enzymatic reactions involved in indole-3-acetic acid (IAA) production via IPyA are different than those previously postulated, and a new and testable model for how IAA is produced in plants is needed. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2012
Number of the records: 1