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Preconceptional paternal glycidamide exposure affects embryonic gene expression: Single embryo gene expression study following in vitro fertilization
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SYSNO ASEP 0371924 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Preconceptional paternal glycidamide exposure affects embryonic gene expression: Single embryo gene expression study following in vitro fertilization Author(s) Brevik, A.. (NO)
Rusňáková, Vendula (BTO-N)
Duale, N. (NO)
Slagsvold, H.H. (NO)
Olsen, A.-K. (NO)
Storeng, R. (NO)
Kubista, Mikael (BTO-N) RID
Brunborg, G. (NO)
Lindeman, B. (NO)Source Title Reproductive Toxicology. - : Elsevier - ISSN 0890-6238
Roč. 32, č. 4 (2011), s. 463-471Number of pages 9 s. Language eng - English Country NO - Norway Keywords Single-cell gene expression ; Glycidamide ; Acrylamide Subject RIV EB - Genetics ; Molecular Biology R&D Projects IAA500520809 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) CEZ AV0Z50520701 - BTO-N (2007-2013) UT WOS 000297716900010 DOI 10.1016/j.reprotox.2011.09.005 Annotation Recognition of early determinants of disease onset has sparked an interest in paternally transmitted factors and their impact on the developing embryo. Acrylamide (AA),a widely distributed xenobiotic compound,is converted to its active metabolite glycidamide (GA) by the CYP2E1 enzyme.Based on its capacity to induce dominant lethal mutations,we hypothesized that paternal GA exposure would have a negative impact on embryonic genome activation,via GA-DNA and protamine adducts persisting in the fertilizing sperm. Using a combination of in vitro fertilization (IVF) techniques and RT-qPCR single embryo gene expression (SEGE), we studied the expression of key DNA repair genes and genes important for embryo development, at the 1-, 2-, 4- and 8-cell stage of the developing mouse embryo. Compared to controls paternal GA-exposure gave rise to an altered pattern of embryonic gene expression, with an initial reduced expression at early stages followed by increased expression at the 8-cell stage. Workplace Institute of Biotechnology Contact Monika Kopřivová, Monika.Koprivova@ibt.cas.cz, Tel.: 325 873 700 Year of Publishing 2012
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