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Tools for genetic manipulations in Corynebacterium glutamicum and their applications
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SYSNO ASEP 0370930 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Tools for genetic manipulations in Corynebacterium glutamicum and their applications Author(s) Nešvera, Jan (MBU-M) RID
Pátek, Miroslav (MBU-M) RID, ORCIDSource Title Applied Microbiology and Biotechnology. - : Springer - ISSN 0175-7598
Roč. 90, č. 5 (2011), s. 1641-1654Number of pages 14 s. Language eng - English Country DE - Germany Keywords Corynebacterium glutamicum ; Plasmid vectors ; Promoters Subject RIV EE - Microbiology, Virology R&D Projects GC204/09/J015 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z50200510 - MBU-M (2005-2011) UT WOS 000290580400004 DOI 10.1007/s00253-011-3272-9 Annotation Corynebacterium glutamicum is an important industrial producer of various amino acids with great potential for the production of other metabolites. The complete genome sequences of two C. glutamicum strains were determined and the use of genome-based approaches (transcriptomics, proteomics, metabolomics, and fluxomics) provided large amounts of data on the metabolism of this bacterium and its regulation. Many tools for genetic manipulations in C. glutamicum have been developed and used for the analysis of gene functions as well as for the construction and improvement of production strains. These tools include various types of plasmid vectors (cloning, promoter-probe, and expression vectors), DNA transfer methods, cloning heterologous genes, introducing protein secretion systems and gene replacement and genome rearrangement methods Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2012
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