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Comparison of new ELISA method with established SDS-PAGE method for determination of muscle myosin heavy chain isoforms
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SYSNO ASEP 0370898 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Comparison of new ELISA method with established SDS-PAGE method for determination of muscle myosin heavy chain isoforms Author(s) Říčný, Jan (FGU-C)
Soukup, Tomáš (FGU-C) RIDSource Title Physiological Research. - : Fyziologický ústav AV ČR, v. v. i. - ISSN 0862-8408
Roč. 60, č. 6 (2011), s. 899-904Number of pages 6 s. Language eng - English Country CZ - Czech Republic Keywords muscle fiber types ; myosin heavy chains ; SDS-PAGE ; immunoreactions ; thyroid hormones ; ELISA Subject RIV EA - Cell Biology R&D Projects GA304/08/0256 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z50110509 - FGU-C (2005-2011) UT WOS 000299021500007 Annotation We have developed a new method for quantitative determination of myosin heavy chain (MyHC) isoforms taking advantage of immunocytochemical differences, based on ELISA principle. We compared compared it with the SDS-PAGE method at the same sample of adult female inbred Lewis strain euthyroid, hyperthyroid and hypothyroid rats. Our results showed that ELISA can be used for a “semi-quantitative” or a “comparative” measurement of MyHC isoforms in multiple muscle samples, but that it is not more exact or faster compared to the SDS-PAGE Workplace Institute of Physiology Contact Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400 Year of Publishing 2012
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