Number of the records: 1

Efficient biolistic transformation of the moss Physcomitrella patens

SYSNO ASEP	0370792
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Efficient biolistic transformation of the moss Physcomitrella patens
Author(s)	Šmídková, Markéta (UEB-Q) Holá, M. (CZ) Angelis, Karel (UEB-Q)_{RID, ORCID}
Source Title	Biologia Plantarum. - : Ústav experimentální botaniky AV ČR, v. v. i. - ISSN 0006-3134 Roč. 54, č. 4 (2010), s. 777-780
Number of pages	4 s.
Language	eng - English
Country	NL - Netherlands
Keywords	Helios biolistic gun ; moss protonemal tissue ; particle size
Subject RIV	EI - Biotechnology ; Bionics
R&D Projects	GA521/04/0971 GA ČR - Czech Science Foundation (CSF)
	IBS5038304 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR)
	1M0505 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	LC06004 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
CEZ	AV0Z50380511 - UEB-Q (2005-2011)
UT WOS	000282125000033
DOI	10.1007/s10535-010-0141-9
Annotation	High rates of homologous recombination (HR) in comparison to other plants make the moss Physcomitrella patens an attractive model organism for genetic studies as well as biotechnological applications. We describe a simple protocol for the efficient biolistic transformation of protonemal tissue with minimum tissue handling steps. The transformation efficiency depends on the biolistic conditions. The bombardment of tissue with 1 mu m gold particles yielded between 20 and 40 stable transformants per 1 mu g of DNA. Transformation with circular plasmids generates higher frequencies of random transgene integration, whereas linear plasmids are more efficient in generating gene-targeted insertions.
Workplace	Institute of Experimental Botany
Contact	David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469
Year of Publishing	2012

Number of the records: 1