Number of the records: 1

Tail-labelling of DNA probes using modified deoxynucleotide triphosphates and terminal deoxynucleotidyl tranferase. Application in electrochemical DNA hybridization and protein-DNA binding assays

SYSNO ASEP	0370713
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Tail-labelling of DNA probes using modified deoxynucleotide triphosphates and terminal deoxynucleotidyl tranferase. Application in electrochemical DNA hybridization and protein-DNA binding assays
Author(s)	Horáková Brázdilová, Petra (BFU-R) Macíčková-Cahová, Hana (UOCHB-X)_{RID, ORCID} Pivoňková, Hana (BFU-R)_ORCID Špaček, Jan (BFU-R)_ORCID Havran, Luděk (BFU-R)_{RID, ORCID} Hocek, Michal (UOCHB-X)_{RID, ORCID} Fojta, Miroslav (BFU-R)_{RID, ORCID}
Number of authors	7
Source Title	Organic & Biomolecular Chemistry. - : Royal Society of Chemistry - ISSN 1477-0520 Roč. 9, č. 5 (2011), s. 1366-1371
Number of pages	6 s.
Language	eng - English
Country	GB - United Kingdom
Keywords	DNA tail-labelling ; protein-DNA binding ; DNA hybridization
Subject RIV	BO - Biophysics
R&D Projects	LC06035 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	LC512 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	IAA400040901 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR)
CEZ	AV0Z50040507 - BFU-R (2005-2011)
	AV0Z50040702 - BFU-R (2007-2013)
	AV0Z40550506 - UOCHB-X (2005-2011)
UT WOS	000287368800014
DOI	10.1039/c0ob00856g
Annotation	A simple approach to DNA tail-labelling using terminal deoxynucleotidyl transferase and modified deoxynucleoside triphosphates is presented. We demonstrated a perfect discrimination between complementary and non-complementary target DNAs sequences by tail-labelled hybridization probes as well as the ability of tumour suppressor p53 protein to recognize a specific binding site within tail-labelled DNA.
Workplace	Institute of Biophysics
Contact	Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244
Year of Publishing	2012

Number of the records: 1