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Nuclear gamma-tubulin associates with nucleoli and interacts with tumor suppressor protein C53
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SYSNO ASEP 0370649 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Nuclear gamma-tubulin associates with nucleoli and interacts with tumor suppressor protein C53 Author(s) Hořejší, Barbora (UMG-J)
Vinopal, Stanislav (UMG-J)
Sládková, Vladimíra (UMG-J)
Dráberová, Eduarda (UMG-J) RID, ORCID
Sulimenko, Vadym (UMG-J) RID, ORCID
Sulimenko, Tetyana (UMG-J)
Vosecká, Věra (UMG-J)
Philimonenko, Anatoly (UMG-J)
Hozák, Pavel (UMG-J) RID, ORCID
Katsetos, C.D. (US)
Dráber, Pavel (UMG-J) RID, ORCIDSource Title Journal of Cellular Physiology. - : Wiley - ISSN 0021-9541
Roč. 227, č. 1 (2012), s. 367-382Number of pages 16 s. Language eng - English Country US - United States Keywords nucleolus ; gamma-tubulin ; C53 Subject RIV EB - Genetics ; Molecular Biology R&D Projects GA204/09/1777 GA ČR - Czech Science Foundation (CSF) GD204/09/H084 GA ČR - Czech Science Foundation (CSF) GAP302/10/1701 GA ČR - Czech Science Foundation (CSF) LC545 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) KAN200520701 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) 1M0506 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) CEZ AV0Z50520514 - UMG-J (2005-2011) UT WOS 000297942500039 DOI https://doi.org/10.1002/jcp.22772 Annotation Immunolocalization and siRNAi cell fractionation plus expression of FLAG-tagged constructs showed presence of γ-tubulin in nucleoli of diverse mammalian interphase cells. It was found outside fibrillar centers of ribosomal DNA transcription, associated with nucleolar remnants after nuclear envelope breakdown and translocated to nucleoli during mitosis. Leptomycin B cell pretreatment did not affect nuclear γ-tubulin distribution, making unlikely rapid active transport of γ-tubulin via nuclear pores into the nucleus. This was confirmed by heterokaryon assay and time-lapse imaging of photoconvertible protein Dendra2 tagged to γ-tubulin. Further experiments revealed and confirmed association of γ-tubulin with tumor suppressor protein C53 located in many subcellular compartments including nucleoli. γ-Tubulin overexpression overcame C53 block of DNA damage G(2)/M checkpoint activation. Beside its known role in microtubule nucleation γ-tubulin may thus also have nuclear-specific function(s). Workplace Institute of Molecular Genetics Contact Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Year of Publishing 2013
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