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Nuclear gamma-tubulin associates with nucleoli and interacts with tumor suppressor protein C53

SYSNO ASEP	0370649
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Nuclear gamma-tubulin associates with nucleoli and interacts with tumor suppressor protein C53
Author(s)	Hořejší, Barbora (UMG-J) Vinopal, Stanislav (UMG-J) Sládková, Vladimíra (UMG-J) Dráberová, Eduarda (UMG-J)_{RID, ORCID} Sulimenko, Vadym (UMG-J)_{RID, ORCID} Sulimenko, Tetyana (UMG-J) Vosecká, Věra (UMG-J) Philimonenko, Anatoly (UMG-J) Hozák, Pavel (UMG-J)_{RID, ORCID} Katsetos, C.D. (US) Dráber, Pavel (UMG-J)_{RID, ORCID}
Source Title	Journal of Cellular Physiology. - : Wiley - ISSN 0021-9541 Roč. 227, č. 1 (2012), s. 367-382
Number of pages	16 s.
Language	eng - English
Country	US - United States
Keywords	nucleolus ; gamma-tubulin ; C53
Subject RIV	EB - Genetics ; Molecular Biology
R&D Projects	GA204/09/1777 GA ČR - Czech Science Foundation (CSF)
	GD204/09/H084 GA ČR - Czech Science Foundation (CSF)
	GAP302/10/1701 GA ČR - Czech Science Foundation (CSF)
	LC545 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	KAN200520701 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR)
	1M0506 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
CEZ	AV0Z50520514 - UMG-J (2005-2011)
UT WOS	000297942500039
DOI	10.1002/jcp.22772
Annotation	Immunolocalization and siRNAi cell fractionation plus expression of FLAG-tagged constructs showed presence of γ-tubulin in nucleoli of diverse mammalian interphase cells. It was found outside fibrillar centers of ribosomal DNA transcription, associated with nucleolar remnants after nuclear envelope breakdown and translocated to nucleoli during mitosis. Leptomycin B cell pretreatment did not affect nuclear γ-tubulin distribution, making unlikely rapid active transport of γ-tubulin via nuclear pores into the nucleus. This was confirmed by heterokaryon assay and time-lapse imaging of photoconvertible protein Dendra2 tagged to γ-tubulin. Further experiments revealed and confirmed association of γ-tubulin with tumor suppressor protein C53 located in many subcellular compartments including nucleoli. γ-Tubulin overexpression overcame C53 block of DNA damage G(2)/M checkpoint activation. Beside its known role in microtubule nucleation γ-tubulin may thus also have nuclear-specific function(s).
Workplace	Institute of Molecular Genetics
Contact	Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217
Year of Publishing	2013

Number of the records: 1