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Analysis of two abundant, highly related satellites in the allotetraploid Nicotiana arentsii using double-strand conformation polymorphism analysis and sequencing

SYSNO ASEP	0370431
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Analysis of two abundant, highly related satellites in the allotetraploid Nicotiana arentsii using double-strand conformation polymorphism analysis and sequencing
Author(s)	Matyášek, Roman (BFU-R)_ORCID Fulneček, Jaroslav (BFU-R) Leitch, A.R. (GB) Kovařík, Aleš (BFU-R)_{RID, ORCID}
Number of authors	4
Source Title	New Phytologist - ISSN 0028-646X Roč. 192, č. 3 (2011), s. 747-759
Number of pages	13 s.
Language	eng - English
Country	US - United States
Keywords	DNA-curvature ; subtelomeric satellite repeats ; allopolyploidy
Subject RIV	BO - Biophysics
R&D Projects	GA206/09/1751 GA ČR - Czech Science Foundation (CSF)
	GAP501/10/0208 GA ČR - Czech Science Foundation (CSF)
	LC06004 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
CEZ	AV0Z50040507 - BFU-R (2005-2011)
	AV0Z50040702 - BFU-R (2007-2013)
UT WOS	000296850800020
DOI	10.1111/j.1469-8137.2011.03827.x
Annotation	In allotetraploid Nicotiana arentsii, we examined the fate of homologous subtelomeric satellites NUNSSP and NWISSP inherited from diploid progenitors N. undulata and N. wigandioides, respectively. While the NUNSSP monomers were highly homogeneous in nucleotide sequences, the NWISSP monomers formed two separate clades. Likewise, the genomic NUNSSP monomers showed less DNA conformation heterogeneity than NWISSP monomers, with distinct conformations. Sequence, structural and chromosomal features of the parental satellites were faithfully inherited by N. arentsii. Our study revealed that inter-genomic homogenisation of subtelomeric satellite repeats does not occur in N. arentsii allotetraploid. We propose that the sequence and structural divergence of subtelomeric satellites may render allopolyploid chromosomes less vulnerable to intergenomic exchanges.
Workplace	Institute of Biophysics
Contact	Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244
Year of Publishing	2012

Number of the records: 1