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The influence of monovalent cations on trimeric G protein Gi1alfa activity in HEK293 cells stably expressing DOR-Gi1alfa (Cys351-Ile351) fusion protein

SYSNO ASEP	0370263
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	The influence of monovalent cations on trimeric G protein Gi1alfa activity in HEK293 cells stably expressing DOR-Gi1alfa (Cys351-Ile351) fusion protein
Author(s)	Vošahlíková, Miroslava (FGU-C)_{RID, ORCID, SAI} Svoboda, Petr (FGU-C)_{RID, ORCID}
Source Title	Physiological Research. - : Fyziologický ústav AV ČR, v. v. i. - ISSN 0862-8408 Roč. 60, č. 3 (2011), s. 541-547
Number of pages	7 s.
Language	eng - English
Country	CZ - Czech Republic
Keywords	delta-opioid receptor (DOR) ; monovalent ions ; G(i)1alfa protein
Subject RIV	CE - Biochemistry
R&D Projects	IAA500110606 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR)
	LC554 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	GD305/08/H037 GA ČR - Czech Science Foundation (CSF)
CEZ	AV0Z50110509 - FGU-C (2005-2011)
UT WOS	000293138600015
Annotation	The effect of monovalent cations on trimeric G protein Gi1alfa was measured at equimolar concentration of chloride anion in pertussis-toxin (PTX)-treated HEK293 cells stably expressing PTX-insensitive DOR-Gi1alfa (Cys351-Ile351) fusion protein by high-affinity [35S]GTPgamaS binding assay. The high basal level of binding was detected in absence of DOR agonist and monovalent ions and this high level was inhibited with the order of: Naplus je větší než Kplus je větší nez Liplus. The inhibition by monovalent ions was reversed by increasing concentrations of DOR agonist DADLE. The maximum DADLE response was also highest for sodium and decreased in the order of: Naplus je větší než Kplus rovná se Liplus. Our data indicate i) an inherently high activity of trimeric G protein Gi1alfa when expressed within DOR-Gi1alfa fusion protein and determined in the absence of monovalent cations, ii) preferential sensitivity of DOR-Gi1alfa to sodium as far as maximum of agonist response is involved
Workplace	Institute of Physiology
Contact	Lucie Trajhanová, lucie.trajhanova@fgu.cas.cz, Tel.: 241 062 400
Year of Publishing	2012

Number of the records: 1