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Stable Agrobacterium-mediated transformation of Norway spruce embryogenic tissues using somatic embryo explants

SYSNO ASEP	0370255
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Ostatní články
Title	Stable Agrobacterium-mediated transformation of Norway spruce embryogenic tissues using somatic embryo explants
Author(s)	Pavingerová, Daniela (BC-A) Bříza, Jindřich (BC-A)_RID Niedermeierová, Hana (BC-A) Vlasák, Josef (BC-A)_{RID, ORCID}
Number of authors	4
Source Title	Journal of Forest Science. - : Czech Academy of Agricultural Sciences - ISSN 1212-4834 Roč. 57, č. 7 (2011), s. 277-280
Number of pages	4 s.
Language	eng - English
Country	CZ - Czech Republic
Keywords	Agrobacterium tumefaciens ; genetic engineering ; GUS activity ; Picea abies (L.) Karst
Subject RIV	EB - Genetics ; Molecular Biology
R&D Projects	QH71290 GA MZe - Ministry of Agriculture (MZe)
CEZ	AV0Z50510513 - UMBR-M, BC-A (2005-2011)
Annotation	In conifers and other plants with long reproductive cycles, transformed embryogenic calli can serve as a convenient source of plant material for the testing insecticidal or fungicidal transgene efficiency. In this report, transgenic embryogenic callus was obtained after transformation of somatic embryos of Norway spruce (Picea abies) by Agrobacterium tumefaciens with the gus-intron chimeric gene. The stable integration of transgenes was confirmed by PCR and Southern hybridization. The transformation was successful only with suitable embryogenic line sensitive to Agrobacterium. From nine embryogenic lines tested only one gave transgenic callus
Workplace	Biology Centre (since 2006)
Contact	Dana Hypšová, eje@eje.cz, Tel.: 387 775 214
Year of Publishing	2012

Number of the records: 1