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Regulation of Src family kinases involved in T cell receptor signaling by protein-tyrosine phosphatase CD148
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SYSNO ASEP 0370118 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Regulation of Src family kinases involved in T cell receptor signaling by protein-tyrosine phosphatase CD148 Author(s) Štěpánek, Ondřej (UMG-J) RID, ORCID
Kalina, T. (CZ)
Dráber, Peter (UMG-J)
Skopcová, Tereza (UMG-J)
Svojgr, K. (CZ)
Angelisová, Pavla (UMG-J) RID
Hořejší, Václav (UMG-J) RID
Weiss, A. (US)
Brdička, Tomáš (UMG-J) RIDSource Title Journal of Biological Chemistry. - : Elsevier - ISSN 0021-9258
Roč. 286, č. 25 (2011), s. 22101-22112Number of pages 12 s. Language eng - English Country US - United States Keywords CD148 ; tyrosine phosphatase ; Src family kinases Subject RIV EB - Genetics ; Molecular Biology R&D Projects 2B06064 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) 1M0506 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) CEZ AV0Z50520514 - UMG-J (2005-2011) UT WOS 000291719900016 DOI 10.1074/jbc.M110.196733 Annotation CD148 is a receptor-like protein-tyrosine phosphatase inhibiting transduction of signals in non-hematopoietic cells. We describe striking differences in CD148 expression between human and murine thymocyte subsets, the only unifying feature being the absence of CD148 during the positive selection when the major developmental block occurs under CD45 deficiency. CD148 has both activating and inhibitory effects on the SFKs involved in TCR signaling. In the absence of CD45, activating effects prevail, resulting in functional complementation of CD45 deficiency in human T cell lines. This is independent of the tyrosines in the CD148 C-terminal tail, contradicting the recently proposed phosphotyrosine displacement model as a mechanism of SFK activation by CD148. Differential effects of CD148 in T cells and other leukocyte subsets cannot be explained by the CD148 inability to activate T cell SFKs but rather by its dual inhibitory/activatory function and specific expression pattern. Workplace Institute of Molecular Genetics Contact Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Year of Publishing 2012
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