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Btk is a positive regulator in the TREM-1/DAP12 signaling pathway
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SYSNO ASEP 0370093 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Btk is a positive regulator in the TREM-1/DAP12 signaling pathway Author(s) Ormsby, Tereza (UMG-J)
Schlecker, E. (DE)
Ferdin, J. (DE)
Tessarz, A.S. (DE)
Angelisová, Pavla (UMG-J) RID
Koprulu, A.D. (AT)
Borte, M. (DE)
Warnatz, K. (DE)
Schulze, I. (DE)
Ellmeier, W. (DE)
Hořejší, Václav (UMG-J) RID
Cerwenka, A. (DE)Source Title Blood. - : American Society of Hematology - ISSN 0006-4971
Roč. 118, č. 4 (2011), s. 936-945Number of pages 10 s. Language eng - English Country US - United States Keywords TREM-1 ; DAP-12 ; Btk Subject RIV EB - Genetics ; Molecular Biology R&D Projects 1M0506 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) CEZ AV0Z50520514 - UMG-J (2005-2011) UT WOS 000293221700020 DOI 10.1182/blood-2010-11-317016 Annotation TREM-1 receptor has been implicated in the production of proinflammatory cytokines and chemokines during bacterial infection and sepsis. For downstream signal transduction, TREM-1 is coupled to the ITAM-containing adaptor DAP12. We demonstrate that Bruton tyrosine kinase (Btk), becomes phosphorylated upon TREM-1 triggering. In cell lines, in which expression of Btk was diminished by shRNA-mediated knockdown, phosphorylation of Erk1/2 and PLCγ1 and Ca²+ mobilization were reduced after TREM-1 stimulation. TREM-1-induced production of the pro-inflammatory cytokines, TNF-α and IL-8, and up-regulation of activation/differentiation cell surface markers were impaired in Btk knockdown cells.Intact membrane localization and a functional kinase domain were required for TREM-1-mediated signaling. After TREM-1 engagement, TNF-α production by PBMCs was reduced in the patients suffering from XLA, a disease caused by mutations in the BTK gene. Workplace Institute of Molecular Genetics Contact Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Year of Publishing 2012
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