Number of the records: 1

Cyclosporine A-loaded and stem cell-seeded electrospun nanofibers for cell-based therapy and local immunosuppression

SYSNO ASEP	0370057
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Cyclosporine A-loaded and stem cell-seeded electrospun nanofibers for cell-based therapy and local immunosuppression
Author(s)	Holáň, Vladimír (UMG-J) Chudíčková, Milada (UMG-J) Trošan, Peter (UMG-J) Svobodová, Eliška (UMG-J) Krulová, Magdalena (UMG-J) Kubinová, Šárka (UEM-P)_{RID, ORCID} Syková, Eva (UEM-P)_RID Širc, Jakub (UMCH-V)_{RID, ORCID} Michálek, Jiří (UMCH-V)_{RID, ORCID} Juklíčková, M. (CZ) Munzarová, M. (CZ) Zajícová, Alena (UMG-J)
Source Title	Journal of Controlled Release. - : Elsevier - ISSN 0168-3659 Roč. 156, č. 3 (2011), s. 406-412
Number of pages	7 s.
Language	eng - English
Country	NL - Netherlands
Keywords	nanofibers ; immunosuppression ; cell transfer
Subject RIV	EC - Immunology
R&D Projects	KAN200520804 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR)
	GAP304/11/0653 GA ČR - Czech Science Foundation (CSF)
	GAP301/11/1568 GA ČR - Czech Science Foundation (CSF)
	GD310/08/H077 GA ČR - Czech Science Foundation (CSF)
	1M0506 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
CEZ	AV0Z50390512 - UEM-P (2005-2011)
	AV0Z40500505 - UMCH-V (2005-2011)
	AV0Z50520514 - UMG-J (2005-2011)
UT WOS	000298555000017
DOI	10.1016/j.jconrel.2011.07.022
Annotation	The authors described preparation and construction of a novel form of nanofiber scaffold which is loaded with immunosuppressive drug cyclosporin A (CsA). The incorporation of CsA did not influence the diameter, shape, porosity or uniformity of the nanofibers. This scaffold has an optimal kinetic of drug release and can serve for a local immunosuppression of immune reactions. The covering of skin allografts with CsA-loaded nanofibers significantly attenuated the expression of the genes for the proinflammatory cytokines. The CsA-containing nanofibers can be seeded with stem cells and thus can be used for cell transfer in regenerative medicine. The results suggest that CsA-loaded electrospun nanofibers prepared from the biodegradable polymer poly(L-lactic acid) can serve as effective drug carriers for the local/topical suppression of an inflammatory reaction and simultaneously as scaffolds for cell transfer for tissue repair and regeneration.
Workplace	Institute of Molecular Genetics
Contact	Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217
Year of Publishing	2012

Number of the records: 1