Number of the records: 1
Comparison of LC Columns Packed with 2.6 lm Core-Shell and Sub-2 lm Porous Particles for Gradient Separation of Antibiotics
- 1.
SYSNO ASEP 0369992 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Comparison of LC Columns Packed with 2.6 lm Core-Shell and Sub-2 lm Porous Particles for Gradient Separation of Antibiotics Author(s) Tylová, Tereza (MBU-M)
Kameník, Zdeněk (MBU-M) RID, ORCID
Flieger, Miroslav (MBU-M) ORCID
Olšovská, Jana (MBU-M)Source Title Chromatographia - ISSN 0009-5893
Roč. 74, 1-2 (2011), s. 19-27Number of pages 9 s. Language eng - English Country DE - Germany Keywords Column liquid chromatography ; Sub-2 mu m particles ; Acquity BEH C18 column Subject RIV EE - Microbiology, Virology R&D Projects 1M06011 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) CEZ AV0Z50200510 - MBU-M (2005-2011) UT WOS 000291745400003 DOI 10.1007/s10337-011-2021-7 Annotation The recently introduced Kinetex C18 column packed with core-shell 2.6 lm particles is declared to provide similar efficiency and short analysis as Acquity BEH C18 column with 1.7 lm porous particles. Unlike Acquity BEH C18 column, Kinetex C18 column exhibited lower column backpressure making this column compatible to conventional LC systems. The performance of Kinetex C18 column (2.1 9 50 mm) and Acquity BEH C18 column (2.1 9 50 mm) for gradient separation of tetracyclines under acidic conditions (oxytetracycline, tetracycline, chlortetracycline, and doxycycline) and macrolides under alkaline conditions (tylosin, clarithromycin, roxithromycin, and carbomycin) was studied. The columns were compared by evaluation of their experimental peak capacity and its dependence on linear velocity and gradient slope Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2012
Number of the records: 1