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Evidence for the presence of proteolytically active secreted aspartic proteinase 1 of Candida parapsilosis in the cell wall

SYSNO ASEP	0369816
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Evidence for the presence of proteolytically active secreted aspartic proteinase 1 of Candida parapsilosis in the cell wall
Author(s)	Vinterová, Zuzana (UOCHB-X) Šanda, Miloslav (UOCHB-X) Dostál, Jiří (UOCHB-X)_{RID, ORCID} Hrušková-Heidingsfeldová, Olga (UOCHB-X)_{RID, ORCID} Pichová, Iva (UOCHB-X)_{RID, ORCID}
Number of authors	5
Source Title	Protein Science. - : Wiley - ISSN 0961-8368 Roč. 20, č. 12 (2011), s. 2004-2012
Number of pages	9 s.
Language	eng - English
Country	US - United States
Keywords	Candida parapsilosis ; secreted aspartic proteinases ; Sapp1p ; cell wall ; biotin ; proteolytic activity
Subject RIV	CE - Biochemistry
R&D Projects	LC531 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	GA310/09/1945 GA ČR - Czech Science Foundation (CSF)
CEZ	AV0Z40550506 - UOCHB-X (2005-2011)
UT WOS	000297462400006
DOI	10.1002/pro.744
Annotation	We found that Sapp1p of C. parapsilosis displays enzyme activity prior to secretion, and therefore, it is probably fully folded within the upper layer of the cell wall. The positioning of cell surface-associated Sapp1p was detected by cell wall protein labeling using biotinylation agents, extraction of cell wall proteins by beta-mercaptoethanol, immunochemical detection, and mass spectrometry analysis. The retaining of Sapp1p in the cell wall is not a totally random process and pathogenic yeasts might use this cell-associated proteinase activity to enhance degradation of appropriate substrates.
Workplace	Institute of Organic Chemistry and Biochemistry
Contact	asep@uochb.cas.cz ; Kateřina Šperková, Tel.: 232 002 584 ; Viktorie Chládková, Tel.: 232 002 434
Year of Publishing	2012

Number of the records: 1