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High-level expression of soluble form of mouse natural killer cell receptor NKR-P1C(B6) in Escherichia coli
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SYSNO ASEP 0369717 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title High-level expression of soluble form of mouse natural killer cell receptor NKR-P1C(B6) in Escherichia coli Author(s) Rozbeský, Daniel (MBU-M)
Kavan, Daniel (MBU-M) RID, ORCID
Chmelík, Josef (MBU-M) RID, ORCID
Novák, Petr (MBU-M) RID, ORCID
Vaněk, Ondřej (MBU-M)
Bezouška, Karel (MBU-M)Source Title Protein Expression and Purification. - : Elsevier - ISSN 1046-5928
Roč. 77, č. 2 (2011), s. 178-184Number of pages 7 s. Language eng - English Country US - United States Keywords Natural killer cell ; NKR-P1C receptor ; NK1.1 antigen Subject RIV EC - Immunology R&D Projects GA303/09/0477 GA ČR - Czech Science Foundation (CSF) GD305/09/H008 GA ČR - Czech Science Foundation (CSF) GAP207/10/1040 GA ČR - Czech Science Foundation (CSF) IAA500200620 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) LC07017 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LC545 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) 1M0505 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) CEZ AV0Z50200510 - MBU-M (2005-2011) UT WOS 000288591900008 DOI 10.1016/j.pep.2011.01.013 Annotation Mouse NKR-P1C(B6) receptor corresponding to NK1.1 alloantigen is one of the most widespread surfacemarkers of mouse NK and NKT cells in C57BL/6 mice detected by monoclonal antibody PK136. Althoughfunctional studies revealed the ability of this receptor to activate both natural killing and production ofcytokines upon antibody crosslinking, the ligand for NKR-P1C(B6) remains unknown. In order to initiate ligand identification, structural studies, and epitope mapping experiments, we developed a simple and efficient expression and purification protocol allowing to produce large amounts of pure soluble monomeric mouse NKR-P1C(B6). Our protein encompassed approximately half of the stalk region and the entire C-terminal globular ligand binding domain Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2012
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