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Asbestos exposure affects poly(ADP-ribose) polymerase-1 activity: role in asbestos-induced carcinogenesis
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SYSNO ASEP 0369504 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Asbestos exposure affects poly(ADP-ribose) polymerase-1 activity: role in asbestos-induced carcinogenesis Author(s) Tomasetti, M. (IT)
Amati, M. (IT)
Nocchi, L. (IT)
Saccucci, F. (IT)
Strafella, E. (IT)
Tarquini, L.M. (IT)
Carbonari, D. (IT)
Alleva, R. (IT)
Borghi, B. (IT)
Neužil, Jiří (BTO-N) RID
Bracci, M. (IT)
Santarelli, L. (IT)Source Title MUTAGENESIS - ISSN 0267-8357
Roč. 26, č. 5 (2011), s. 585-591Number of pages 7 Language eng - English Country GB - United Kingdom Keywords Asbestos exposure ; poly(ADP-ribose) polymerase-1 ; malignant mesothelioma Subject RIV EB - Genetics ; Molecular Biology CEZ AV0Z50520701 - BTO-N (2007-2013) UT WOS 000294554600002 DOI 10.1093/mutage/ger020 Annotation Asbestos is known to induce malignant mesothelioma (MM). It is directly genotoxic by inducing DNA strand breaks. Poly(ADP-ribose) polymerase-1 (PARP1) is a protein with a function as a DNA damage sensor. To determine whether PARP1 is involved in asbestos-induced carcinogenesis, PARP1 expression and activity as well as DNA damage and repair were evaluated in circulating cells of asbestos-exposed subjects, MM patients and controls. Accumulation of the pre-mutagenic 8-hydroxy-2'-deoxyguanosine and elevated PARP1 expression were found both in asbestos-exposed subjects and MM patients. Although PARP1 was highly expressed, its activity was relatively low in circulating cells as well as in MM biopsies. Low DNA repair efficiency was observed in lymphocytes from MM patients. To mimic PARP1 dysfunction, PARP1 expression and activity were induced in immortalised mesothelial cells by their exposure to asbestos in the presence of a PARP1 inhibitor, which resulted in transformation of the cells. Workplace Institute of Biotechnology Contact Monika Kopřivová, Monika.Koprivova@ibt.cas.cz, Tel.: 325 873 700 Year of Publishing 2012
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