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Ligand-binding properties of a juvenile hormone receptor, Methoprene-tolerant

SYSNO ASEP	0369355
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Ligand-binding properties of a juvenile hormone receptor, Methoprene-tolerant
Author(s)	Charles, J.-P. (FR) Iwema, T. (FR) Epa, V. C. (AU) Takaki, Keiko (BC-A) Ryneš, J. (CZ) Jindra, Marek (BC-A)_{RID, ORCID}
Number of authors	6
Source Title	Proceedings of the National Academy of Sciences of the United States of America. - : National Academy of Sciences - ISSN 0027-8424 Roč. 108, č. 52 (2011), s. 21128-21133
Number of pages	6 s.
Language	eng - English
Country	US - United States
Keywords	structure modeling ; insecticide action ; metamorphosis
Subject RIV	EB - Genetics ; Molecular Biology
R&D Projects	GD204/09/H058 GA ČR - Czech Science Foundation (CSF)
	GA204/07/1032 GA ČR - Czech Science Foundation (CSF)
	IAA500960906 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR)
CEZ	AV0Z50070508 - ENTU-I, BC-A (2005-2011)
UT WOS	000298479900054
DOI	10.1073/pnas.1116123109
Annotation	Juvenile hormone (JH) is a terpenoid, a small lipophilic molecule of vital importance to insects. The molecular action of JH has for long decades remained an enigma, because a JH receptor could not be identified. In this study we provide conclusive evidence for the JH receptor role of the bHLH-PAS domain transcription factor Methoprene-tolerant (Met). By testing Met point mutant proteins in vitro, we determined specific amino acids contributing to the high-affinity JH binding. Using mutants unable to bind JH, we demonstrated that interactions of Met with partner proteins require the ligand-binding capacity of Met. Therefore, Met resembles certain nuclear receptors, e. g., of retinoic acid or thyroid hormone, but since Met belongs to a different protein family, it establishes a prototype of a hormonal receptor of a novel class. Our results address a long-standing question of the mechanism of action of juvenile hormone and its insecticidal analogs.
Workplace	Biology Centre (since 2006)
Contact	Dana Hypšová, eje@eje.cz, Tel.: 387 775 214
Year of Publishing	2012

Number of the records: 1