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Limitations of Electronic Energy Transfer in the Determination of Lipid Nanodomain Sizes
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SYSNO ASEP 0369083 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Limitations of Electronic Energy Transfer in the Determination of Lipid Nanodomain Sizes Author(s) Šachl, Radek (UFCH-W) RID, ORCID
Humpolíčková, Jana (UFCH-W) RID
Štefl, Martin (UFCH-W)
Johansson, L. B. A. (SE)
Hof, Martin (UFCH-W) RID, ORCIDSource Title Biophysical Journal. - : Cell Press - ISSN 0006-3495
Roč. 101, č. 11 (2011), L60-L62Number of pages 3 s. Language eng - English Country US - United States Keywords Foerster resonance energy transfer ; nanodomains ; Monte Carlo Setup Subject RIV CF - Physical ; Theoretical Chemistry R&D Projects GAP208/10/1090 GA ČR - Czech Science Foundation (CSF) GAP208/10/0376 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z40400503 - UFCH-W (2005-2011) UT WOS 000297897300002 DOI 10.1016/j.bpj.2011.11.001 Annotation Even though superresolution microscopy indicates that size of plasma membrane rafts is <20 nm, those structures have never been observed. Förster resonance energy transfer (FRET) is therefore still the most powerful optical method for characterization of such domains. In this letter we investigate relation between nanodomain affinity of a donor-acceptor (D/A) pair and the detectable nanodomain size/area. We show that probes with high affinity to the liquid-ordered (Lo) phase are required for detecting domain sizes of a few nanometers, and/or domains that occupy a few percent of the bilayer area. A combination of donors and acceptors that prefer different phases is the more favorable approach. For instance, a D/A pair with the distribution constant of donors KD = 5 and acceptors KA = 0.01 can resolve a broad spectrum of nanodomain sizes. On the other hand, currently available donors and acceptors that prefer the same phase, either the liquid-disordered (Ld) or Lo phase, are not so convenient for determining domain sizes <20 nm. Here the detection limits of FRET experiments employing several commonly used D/A pairs have been investigated. Workplace J. Heyrovsky Institute of Physical Chemistry Contact Michaela Knapová, michaela.knapova@jh-inst.cas.cz, Tel.: 266 053 196 Year of Publishing 2012
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