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Changes in cytokinin levels and metabolism in tobacco (Nicotiana tabacum L.) explants during in vitro shoot organogenesis induced by trans-zeatin and dihydrozeatin

SYSNO ASEP	0368520
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Changes in cytokinin levels and metabolism in tobacco (Nicotiana tabacum L.) explants during in vitro shoot organogenesis induced by trans-zeatin and dihydrozeatin
Author(s)	Klemš, M. (CZ) Slámová, Z. (CZ) Motyka, Václav (UEB-Q)_{RID, ORCID} Malbeck, Jiří (UEB-Q)_{RID, ORCID} Trávníčková, Alena (UEB-Q)_ORCID Macháčková, Ivana (UEB-Q) Holík, Josef (UEB-Q)_{RID, ORCID} Procházka, S. (CZ)
Source Title	Plant Growth Regulation. - : Springer - ISSN 0167-6903 Roč. 65, č. 3 (2011), s. 427-437
Number of pages	11 s.
Language	eng - English
Country	NL - Netherlands
Keywords	Beta-glucosidase ; Cytokinin oxidase/ dehydrogenase ; Dihydrozeatin
Subject RIV	EB - Genetics ; Molecular Biology
R&D Projects	1M06030 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	GAP506/11/0774 GA ČR - Czech Science Foundation (CSF)
	IAA600380701 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR)
CEZ	AV0Z50380511 - UEB-Q (2005-2011)
UT WOS	000297621200001
DOI	10.1007/s10725-011-9612-z
Annotation	The uptake and metabolism of trans-zeatin (Z) and/or dihydrozeatin (DHZ), in correlation with cytokinin oxidase/dehydrogenase (CKX) and glucosidase activity, were studied in leaf segments derived from wild-type (WT) and transgenic (T) tobacco (Nicotiana tabacum L. cv. Petit Havana SR1) during in vitro induction of shoot organogenesis. T explants harbored the maize gene Zm-p60.1-glucosidase. Higher levels of shoot regeneration were observed on T explants in the early stages of cultivation. In WT explants, the content of cytokinin (CK)-O- and N-glucosides increased. In T explants, a higher content of Z-9-riboside and its monophosphate and higher CKX activity during the early stage of cultures were found. A positive correlation was obtained for bioactive CK content and the organogenic response in T explants. Our results indicate a connection between the organogenic capacity of tobacco explants, metabolism of endogenous CKs and uptake of exogenous CKs from the cultivation medium.
Workplace	Institute of Experimental Botany
Contact	David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469
Year of Publishing	2012

Number of the records: 1