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Tumour necrosis factor-alpha increases extravasation of virus particles into tumour tissue by activating the Rho A/Rho kinase pathway
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SYSNO ASEP 0368414 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Tumour necrosis factor-alpha increases extravasation of virus particles into tumour tissue by activating the Rho A/Rho kinase pathway Author(s) Seki, T. (GB)
Carroll, F. (GB)
Illingworth, S. (GB)
Green, N. (GB)
Cawood, R. (GB)
Bachtarzi, H. (GB)
Šubr, Vladimír (UMCH-V) RID, ORCID
Fisher, K. D. (GB)
Seymour, L. W. (GB)Source Title Journal of Controlled Release. - : Elsevier - ISSN 0168-3659
Roč. 156, č. 3 (2011), s. 381-389Number of pages 9 s. Language eng - English Country NL - Netherlands Keywords drug delivery ; adenovirus ; vascular permeability Subject RIV CD - Macromolecular Chemistry CEZ AV0Z40500505 - UMCH-V (2005-2011) UT WOS 000298555000014 DOI 10.1016/j.jconrel.2011.08.022 Annotation Tumour Necrosis Factor alpha (TNF) is a cytokine with known vascular permeabilising activity. It is employed during isolated limb perfusion to enhance delivery of chemotherapeutic drugs into tumour tissue. The use of lytic viruses provides a new approach to cancer treatment that is limited by the low efficiency of extravasation of viral particles into tumours. TNF enhances the delivery of virus particles through the endothelial layer to tumour cells in vitro and in vivo. Intravenous administration of TNF resulted in a 3- to 6-fold increase in EL4 tumour uptake of Evans Blue/Albumin, adenovirus and long-circulating polymer coated adenovirus. Endothelial permeabilisation could be suppressed in vitro and in vivo by Y-27632, a Rho kinase inhibitor, without inhibiting viral infection. TNF enhances the delivery of virus particles into tumours through a Rho A/Rho kinase dependent mechanism and may be a new strategy for increasing the delivery of oncolytic viruses and other therapeutic agents. Workplace Institute of Macromolecular Chemistry Contact Eva Čechová, cechova@imc.cas.cz ; Tel.: 296 809 358 Year of Publishing 2012
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