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Raman microspectroscopy based sensor of algal lipid unsaturation
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SYSNO ASEP 0368397 Document Type C - Proceedings Paper (int. conf.) R&D Document Type Conference Paper Title Raman microspectroscopy based sensor of algal lipid unsaturation Author(s) Samek, Ota (UPT-D) RID, ORCID, SAI
Pilát, Zdeněk (UPT-D) RID, SAI, ORCID
Jonáš, Alexandr (UPT-D) RID, SAI, ORCID
Zemánek, Pavel (UPT-D) RID, SAI, ORCID
Šerý, Mojmír (UPT-D) RID, SAI
Ježek, Jan (UPT-D) RID, ORCID, SAI
Bernatová, S. (CZ)
Nedbal, L. (CZ)
Trtílek, M. (CZ)Number of authors 9 Source Title Optical Sensors 2011 and Photonic Crystal Fibers V (Proceedings of SPIE Vol. 8073). - Bellingham : SPIE, 2011 - ISBN 978-0-8194-8663-9 Pages 80730o:1-6 Number of pages 6 s. Action Optical Sensors 2011 and Photonic Crystal Fibers V Event date 18.04.2011-20.04.2011 VEvent location Praha Country CZ - Czech Republic Event type WRD Language eng - English Country US - United States Keywords Raman spectroscopy ; algal cells ; lipids ; iodine value Subject RIV BH - Optics, Masers, Lasers R&D Projects ED0017/01/01 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) OC08034 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) FR-TI1/433 GA MPO - Ministry of Industry and Trade (MPO) CEZ AV0Z20650511 - UPT-D (2005-2011) UT WOS 000293740400014 Annotation Raman spectroscopy is a powerful tool for chemical analysis. This technique can elucidate fundamental questions about the metabolic processes and intercellular variability on a single cell level. Therefore, Raman spectroscopy can significantly contribute to the study and use of microalgae in systems biology and biofuel technology. Raman spectroscopy can be combined with optical tweezers. We have employed microfluidic system to deliver the sampled microalgae to the Raman-tweezers. This instrument is able to measure chemical composition of cells and to track metabolic processes in vivo, in real-time and label-free making it possible to detect population variability in a wide array of traits. Moreover, employing an active sorting switch, cells can be separated depending on input parameters obtained from Raman spectra. We focus on algal lipids which are promising potential products for biofuel as well as for nutrition. Workplace Institute of Scientific Instruments Contact Martina Šillerová, sillerova@ISIBrno.Cz, Tel.: 541 514 178 Year of Publishing 2012
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