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Characterization of microorganisms using Raman tweezers
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SYSNO ASEP 0368396 Document Type C - Proceedings Paper (int. conf.) R&D Document Type Conference Paper Title Characterization of microorganisms using Raman tweezers Author(s) Samek, Ota (UPT-D) RID, ORCID, SAI
Pilát, Zdeněk (UPT-D) RID, SAI, ORCID
Jonáš, Alexandr (UPT-D) RID, SAI, ORCID
Zemánek, Pavel (UPT-D) RID, SAI, ORCID
Šerý, Mojmír (UPT-D) RID, SAI
Ježek, Jan (UPT-D) RID, ORCID, SAI
Bernatová, S. (CZ)
Nedbal, L. (CZ)
Trtílek, M. (CZ)Number of authors 9 Source Title Optical Trapping and Optical Micromanipulation VIII (Proceedings of SPIE Vol. 8097). - Bellingham : SPIE, 2011 - ISBN 978-0-8194-8707-0 Pages 80970f:1-7 Number of pages 7 s. Action Conference on Optical Trapping and Optical Micromanipulation VIII Event date 21.08.2011-25.08.2011 VEvent location San Diego Country US - United States Event type WRD Language eng - English Country US - United States Keywords Raman spectroscopy ; bacteria ; algal cells ; lipids ; iodine value Subject RIV BH - Optics, Masers, Lasers R&D Projects ED0017/01/01 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) GAP205/11/1687 GA ČR - Czech Science Foundation (CSF) FR-TI1/433 GA MPO - Ministry of Industry and Trade (MPO) CEZ AV0Z20650511 - UPT-D (2005-2011) UT WOS 000296029200010 Annotation The ability to identify and characterize microorganisms (algae, bacteria, eukaryotic cells) from minute sample volumes in a rapid and reliable way is the crucial first step in their classification and characterization. In the light of this challenge related to microorganisms exploitation Raman spectroscopy can be used as a powerful tool for chemical analysis. Raman spectroscopy can elucidate fundamental questions about the metabolic processes and intercellular variability on a single cell level. Moreover, Raman spectroscopy can be combined with optical tweezers and with microfluidic chips to measure nutrient dynamics and metabolism in vivo, in real-time, and label free. We demonstrate the feasibility to employ Raman spectroscopy-based sensor to sort microorganisms (bacteria, algae) according to the Raman spectra. It is now quite feasible to sort algal cells according to the degree of unsaturation (iodine value) in lipid storage bodies. Workplace Institute of Scientific Instruments Contact Martina Šillerová, sillerova@ISIBrno.Cz, Tel.: 541 514 178 Year of Publishing 2012
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