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Activation of Cumulus Cell SMAD2/3 and Epidermal Growth Factor Receptor Pathways Are Involved in Porcine Oocyte-Cumulus Cell Expansion and Steroidogenesis
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SYSNO ASEP 0368348 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Activation of Cumulus Cell SMAD2/3 and Epidermal Growth Factor Receptor Pathways Are Involved in Porcine Oocyte-Cumulus Cell Expansion and Steroidogenesis Author(s) Nagyová, Eva (UZFG-Y) RID, ORCID
Camaioni, A. (IT)
Scsuková, S. (SK)
Mlynarčíková, A. (SK)
Procházka, Radek (UZFG-Y) RID, ORCID
Němcová, Lucie (UZFG-Y) RID
Salustri, A. (IT)Number of authors 7 Source Title Molecular Reproduction and Development - ISSN 1040-452X
Roč. 78, č. 6 (2011), s. 391-402Number of pages 12 s. Language eng - English Country US - United States Keywords MURAL GRANULOSA-CELLS ; IN-VITRO MATURATION ; PREOVULATORY OVARIAN-FOLLICLES Subject RIV EB - Genetics ; Molecular Biology R&D Projects GA523/08/0111 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z50450515 - UZFG-Y (2005-2011) UT WOS 000292500700003 DOI 10.1002/mrd.21312 Annotation Several lines of evidence suggest that in mice the activation of SMAD2/3 signaling by oocyte secreted factors, together with epidermal growth factor receptor (EGFR) activation, is essential to induce cumulus expansion. Here we show that inhibition of EGFR kinase in follicle stimulating hormone (FSH)-stimulated porcine oocyte-cumulus cell complex (OCCs) strongly decreases hyaluronan (HA) synthesis and its retention in the matrix, as well as progesterone synthesis. Although porcine cumulus cells undergo expansion independently of oocytes, we use biochemical and gene expression analyses to show that they do require activation of SMAD2/3 for optimal stimulation of HA synthesis and proteins involved in the organization of this polymer in the expanded matrix. Furthermore, FSH-induced progesterone synthesis by porcine cumulus cells was increased by blocking SMAD2/3 activation. Workplace Institute of Animal Physiology and Genetics Contact Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554 Year of Publishing 2012
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