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Activity of phytate dephosphorylation in cereals, legumes and oilseeds determined by colourimetric and isotachophoretic method
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SYSNO ASEP 0368275 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Activity of phytate dephosphorylation in cereals, legumes and oilseeds determined by colourimetric and isotachophoretic method Author(s) Marounek, Milan (UZFG-Y) RID
Bubancová, I. (CZ)
Podsedníček, M. (CZ)
Lukešová, D. (CZ)
Ramadan, M. A. (UZFG-Y)Number of authors 5 Source Title Journal of Animal and Feed Sciences - ISSN 1230-1388
Roč. 20, č. 3 (2011), s. 472-479Number of pages 8 s. Language eng - English Country PL - Poland Keywords phosphorus ; phytate ; dephosphorylation Subject RIV GM - Food Processing R&D Projects GD525/08/H060 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z50450515 - UZFG-Y (2005-2011) UT WOS 000294933400013 Annotation Activity of phytate dephosphorylation was determined in 26 samples of cereals, legumes, oilseeds and complete diets for poultry using the colourimetric and isotachophoretic method. The former method was based on the colour formed upon the reaction of molybdate with phosphate released from phytate. The latter method was based on measurement of decrease of phytate concentration in the reaction mixture. Rye had by far the highest phytate dephosphorylation activity, followed by wheat and wheat-containing diets. However, for most of the other samples, this activity was low. Phytate dephosphorylation activity as measured by capillary isotachophoresis was numerically lower than activity measured using the molybdate method; however, activities assayed by both methods correlated with statistical significance (r=0.86; P<0.001). The isotachophoretic method may be suitable for phytate dephosphorylation activity assay in feeds with a high content of inorganic phosphate and in samples of digesta and faeces. Workplace Institute of Animal Physiology and Genetics Contact Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554 Year of Publishing 2012
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