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Optimized conditions for mesenchymal stem cells to differentiate into osteoblasts on a collagen/hydroxyapatite matrix

SYSNO ASEP	0368158
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Optimized conditions for mesenchymal stem cells to differentiate into osteoblasts on a collagen/hydroxyapatite matrix
Author(s)	Prosecká, Eva (UEM-P) Rampichová, Michala (UEM-P)_{RID, ORCID} Vojtová, L. (CZ) Tvrdík, D. (CZ) Melčáková, Š. (CZ) Juhásová, Jana (UZFG-Y)_{RID, ORCID} Plencner, Martin (UEM-P) Jakubová, Radka (UEM-P) Jančář, J. (CZ) Nečas, A. (CZ) Kochová, P. (CZ) Klepáček, J. (CZ) Tonar, Z. (CZ) Amler, Evžen (UEM-P)_RID
Source Title	Journal of Biomedical Materials Research. Part A. - : Wiley - ISSN 1549-3296 99A, č. 2 (2011), s. 307-315
Number of pages	9 s.
Language	eng - English
Country	US - United States
Keywords	collagen/HA scaffold ; MSCs ; osteoblasts
Subject RIV	BO - Biophysics
R&D Projects	2B06130 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
	GAP304/10/1307 GA ČR - Czech Science Foundation (CSF)
	IAA500390702 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR)
	7E09088 GA MŠMT - Ministry of Education, Youth and Sports (MEYS)
CEZ	AV0Z50390512 - UEM-P (2005-2011)
	AV0Z50390703 - UEM-P (2007-2013)
	AV0Z50450515 - UZFG-Y (2005-2011)
UT WOS	000295142400018
DOI	10.1002/jbm.a.33189
Annotation	Pulsed laser deposition was proved as a suitable method for hydroxyapatite (HA) coating of coaxial poly-ε-caprolactone/polyvinylalcohol (PCL/PVA) nanofibers. The fibrous morphology of PCL/PVA nanofibers was preserved, if the nanofiber scaffold was coated with a thin layers of HA (200 nm and 400 nm). Increasing thickness of HA, however, resulted in a gradual loss of fibrous character. In addition, biomechanical properties were improved after HA deposition on PCL/PVA nanofibers as the value of Young's moduli of elasticity significantly increased. Cle arly, thin-layer hydroxyapatite deposition on a nanofiber surface stimulated mesenchymal stem cell viability and their differentiation into osteoblasts. The optimal depth of HA was 800 nm.
Workplace	Institute of Experimental Medicine
Contact	Lenka Koželská, lenka.kozelska@iem.cas.cz, Tel.: 241 062 218, 296 442 218
Year of Publishing	2012

Number of the records: 1