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Geranylated flavanone tomentodiplacone B inhibits proliferation of human monocytic leukaemia (THP-1) cells
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SYSNO ASEP 0367955 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Geranylated flavanone tomentodiplacone B inhibits proliferation of human monocytic leukaemia (THP-1) cells Author(s) Kollár, P. (CZ)
Bárta, Tomáš (UEM-P)
Závalová, V. (CZ)
Šmejkal, K. (CZ)
Hampl, Aleš (UEM-P)Source Title British Journal of Pharmacology. - : Wiley - ISSN 0007-1188
Roč. 162, č. 7 (2011), s. 1534-1541Number of pages 8 s. Language eng - English Country GB - United Kingdom Keywords flavonoids ; CDK2 ; antiproliferative effect Subject RIV FR - Pharmacology ; Medidal Chemistry CEZ AV0Z50390703 - UEM-P (2007-2013) AV0Z50390512 - UEM-P (2005-2011) UT WOS 000288018100007 DOI 10.1111/j.1476-5381.2010.01171.x Annotation Paulownia tomentosa is a rich source of geranylated flavanones, some of which we have previously shown to have cytotoxic activity. Cell viability was measured by dye exclusion and proliferation by WST-1 assays; cell cycle was monitored by flow cytometry. Regulatory proteins were assessed by immunoprecipitation and kinase assays, and Western blotting. Tomentodiplacone B had no effect during the first 24 h of cell growth at concentrations between 1 and 2.5 mu M, but inhibited cell growth in a dose-dependent manner at concentrations of 5 mu M or higher. Phase G1 accumulation was associated with down-regulation of cyclin-dependent kinase 2 activity and also protein levels of cyclins E1 and A2. Our study provides the first evidence that TOM B directly inhibits proliferation of human monocytic leukaemia cells, and thus is a potential anticancer agent, preventing leukaemia cells from progressing from G1 phase into DNA synthesis. Workplace Institute of Experimental Medicine Contact Lenka Koželská, lenka.kozelska@iem.cas.cz, Tel.: 241 062 218, 296 442 218 Year of Publishing 2012
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