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Surface Plasmon Resonance Biosensor for Determination of Tetrodotoxin: Prevalidation Study
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SYSNO ASEP 0367829 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Surface Plasmon Resonance Biosensor for Determination of Tetrodotoxin: Prevalidation Study Author(s) Vaisocherová, Hana (URE-Y) RID
Taylor, A. D. (US)
Jiang, S. (US)
Hegnerová, Kateřina (URE-Y)
Vala, Milan (URE-Y) RID
Homola, Jiří (URE-Y) RID
Yakes, B. (US)
Deeds, J. (US)
DeGrasse, S. (US)Number of authors 9 Source Title Journal of Aoac International - ISSN 1060-3271
Roč. 94, č. 2 (2011), s. 596-604Number of pages 9 s. Language eng - English Country US - United States Keywords toxin detection ; biosensor ; validtion Subject RIV JA - Electronics ; Optoelectronics, Electrical Engineering R&D Projects KAN200670701 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) CEZ AV0Z20670512 - URE-Y (2005-2011) UT WOS 000289544500028 EID SCOPUS 79953674477 Annotation A label-free surface plasmon resonance biosensor method was applied to determine tetrodotoxin (TTX) in pufferfish matrixes using an antibody inhibition assay format. A prevalidation study was conducted to demonstrate the assay performance characteristics, such as selectivity, LOD, LOQ, repeatability, reproducibility, and accuracy.The developed method was demonstrated to be capable of detecting TTX in pufferfish matrix standard samples in a broad concentration range (2-9000 ng/mL) with an LOD of 1.5 ng/mL. Workplace Institute of Radio Engineering and Electronics Contact Petr Vacek, vacek@ufe.cz, Tel.: 266 773 413, 266 773 438, 266 773 488 Year of Publishing 2012
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