Number of the records: 1
Osteogenic Differentiation of Miniature Pig Mesenchymal Stem Cells in 2D and 3D Environment
- 1.
SYSNO ASEP 0367748 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Osteogenic Differentiation of Miniature Pig Mesenchymal Stem Cells in 2D and 3D Environment Author(s) Juhásová, Jana (UZFG-Y) RID, ORCID
Juhás, Štefan (UZFG-Y) RID, ORCID
Klíma, Jiří (UZFG-Y) RID, ORCID
Strnádel, Ján (UZFG-Y) RID
Holubová, Monika (UZFG-Y)
Motlík, Jan (UZFG-Y) RID, ORCIDNumber of authors 6 Source Title Physiological Research. - : Fyziologický ústav AV ČR, v. v. i. - ISSN 0862-8408
Roč. 60, č. 3 (2011), s. 559-571Number of pages 13 s. Language eng - English Country CZ - Czech Republic Keywords miniature pig ; mesenchymal stem cells ; cell differentiation Subject RIV EB - Genetics ; Molecular Biology R&D Projects 1M0538 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) 2B06130 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) CEZ AV0Z50450515 - UZFG-Y (2005-2011) UT WOS 000293138600017 Annotation Mesenchymal stem cells (MSCs) have been repeatedly shown to be able to repair bone defects. The aim of this study was to characterize the osteogenic differentiation of miniature pig MSCs and markers of this differentiation in vitro. Flow-cytometrically characterized MSCs were seeded on cultivation plastic (collagen I and vitronectin coated/uncoated) or plasma clot (PC)/plasma-alginate clot (PAC) scaffolds and differentiated in osteogenic medium. During three weeks of differentiation, the formation of nodules and deposition of calcium were visualized by Alizarin Red Staining. In addition, the production of alkaline phosphatase (ALP) activity was quantitatively detected by fluorescence. The expression of osteopontin, osteonectin and osteocalcin were assayed by immunohistochemistry and Western Blot analysis. We revealed a decrease of osteopontin expression in 2D and 3D environment during differentiation. Workplace Institute of Animal Physiology and Genetics Contact Jana Zásmětová, knihovna@iapg.cas.cz, Tel.: 315 639 554 Year of Publishing 2012
Number of the records: 1