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Applications of phasors to in vitro time-resolved fluorescence measurements
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SYSNO ASEP 0367710 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Applications of phasors to in vitro time-resolved fluorescence measurements Author(s) Štefl, Martin (UFCH-W)
James, N. G. (US)
Jameson, D. M. (US)Source Title Analytical Biochemistry. - : Elsevier - ISSN 0003-2697
Roč. 410, č. 1 (2011), s. 62-69Number of pages 8 s. Language eng - English Country US - United States Keywords phasor ; fraquency domain ; fluorescence lifetimes Subject RIV CF - Physical ; Theoretical Chemistry R&D Projects LC06063 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) CEZ AV0Z40400503 - UFCH-W (2005-2011) UT WOS 000286711300010 DOI 10.1016/j.ab.2010.11.010 Annotation The phasor method of treating fluorescence lifetime data provides a facile and convenient approach to characterize lifetime heterogeneity and to detect the presence of excited state reactions such as solvent relaxation and Forster resonance energy transfer. The method uses a plot of M sin(Phi) versus M cos(Phi), where M is the modulation ratio and Phi is the phase angle taken from frequency domain fluorometry. A principal advantage of the phasor method is that it provides a model-less approach to time-resolved data amenable to visual inspection. Although the phasor approach has been recently applied to fluorescence lifetime imaging microscopy, it has not been used extensively for cuvette studies. In the current study, we explore the applications of the method to in vitro samples. The phasors of binary and ternary mixtures of fluorescent dyes demonstrate the utility of the method for investigating complex mixtures. Workplace J. Heyrovsky Institute of Physical Chemistry Contact Michaela Knapová, michaela.knapova@jh-inst.cas.cz, Tel.: 266 053 196 Year of Publishing 2012
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