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Reproductive tissue expression and sperm localization of porcine beta-microseminoprotein
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SYSNO ASEP 0367617 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Reproductive tissue expression and sperm localization of porcine beta-microseminoprotein Author(s) Maňásková-Postlerová, Pavla (BTO-N) ORCID
Davidová, Nina (BTO-N)
Šulc, Miroslav (MBU-M) RID, ORCID
Philimonenko, Anatoly (UMG-J)
Hozák, Pavel (UMG-J) RID, ORCID
Jonáková, Věra (BTO-N) RIDNumber of authors 6 Source Title Cell and Tissue Research - ISSN 0302-766X
Roč. 344, č. 2 (2011), s. 341-353Number of pages 13 s. Language eng - English Country DE - Germany Keywords porcine beta-microseminoprotein ; reproductive organs ; spermatozoa ; immunofluorescence ; sperm acrosome ; boar Subject RIV CE - Biochemistry R&D Projects GA303/09/1285 GA ČR - Czech Science Foundation (CSF) GD523/08/H064 GA ČR - Czech Science Foundation (CSF) NS10009 GA MZd - Ministry of Health (MZ) 1M06011 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LC07017 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) LC545 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) CEZ AV0Z50520701 - BTO-N (2007-2013) AV0Z50520514 - UMG-J (2005-2011) AV0Z50200510 - MBU-M (2005-2011) UT WOS 000290167600016 DOI 10.1007/s00441-011-1149-y Annotation Porcine beta-microseminoprotein (MSP) has been described as a protein characteristic of the prostate; however, we proved its mRNA transcripts in all reproductive organs. In tissue extracts, specific antibody antiMSP recognized a 12-kDa protein band, which was identified by mass spectrometry as MSP, and immunofluorescence displayed the MSP occurrence in epithelial tissue. MSP was localized on epididymal sperm in the acrosomal region and in addition to that on the flagellum of ejaculated sperm. The MSP absence on the surface of capacitated sperm together with the antibody detection of MSP in the sperm acidic extract after in vitro capacitation indicated its acrosomal origin. The MSP sperm localization was confirmed by electron microscopy. The secretion of porcine MSP by different reproductive organs might indicate its multiple roles in the reproductive process, and for the first time in mammalian species, the MSP localization has been shown at different physiological stages of sperm. Workplace Institute of Biotechnology Contact Monika Kopřivová, Monika.Koprivova@ibt.cas.cz, Tel.: 325 873 700 Year of Publishing 2012
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