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Ubiquitin-activating enzyme (UBA1) is required for sperm capacitation, acrosomal exocytosis and sperm-egg coat penetration during porcine fertilization
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SYSNO ASEP 0367613 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Ubiquitin-activating enzyme (UBA1) is required for sperm capacitation, acrosomal exocytosis and sperm-egg coat penetration during porcine fertilization Author(s) Yi, Y.-J. (KR)
Zimmermann, S.W. (US)
Manandhar, G. (US)
Odhiambo, J.F. (US)
Kennedy, C. (US)
Jonáková, Věra (BTO-N) RID
Maňásková-Postlerová, Pavla (BTO-N) ORCID
Sutovsky, M. (US)
Park, C.-S. (US)
Sutovsky, P. (US)Number of authors 10 Source Title International Journal of Andrology. - : WILEY-BLACKWELL - ISSN 0105-6263
Roč. 35, č. 2 (2012), s. 196-210Number of pages 15 s. Language eng - English Country US - United States Keywords acrosome ; capacitation ; fertilization ; ubiqutin Subject RIV CE - Biochemistry R&D Projects GA303/09/1285 GA ČR - Czech Science Foundation (CSF) 1M06011 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) NS10009 GA MZd - Ministry of Health (MZ) CEZ AV0Z50520701 - BTO-N (2007-2013) UT WOS 000301442200012 DOI 10.1111/j.1365-2605.2011.01217.x. Annotation Protein ubiquitination is covalent post-translational modification that targets proteins for proteolysis by the 26S proteasome. The E1-type ubiquitin-activating enzyme (UBA1) is responsible for the initial step of ubiquitin–protein ligation. Proteasomal proteolysis of ubiquitinated spermatozoa and oocyte proteins occurs during mammalian fertilization. UBA1 was detected in boar sperm-acrosomal extracts by Western blotting (WB) and by IMF in the acrosomal caps. A specific UBA1 inhibitor, PYR-41, altered the outer acrosomal membrane during sperm capacitation and reduced fertilization rates during in vitro fertilization. WB with antiphosphotyrosine antibodies and antibodies against acrosomal proteins SPINK2 (acrosin inhibitor) and AQN1 (spermadhesin) revealed that the capacitationinduced modification of those proteins was altered by PYR-41. It appears that de novo protein ubiquitination involving UBA1 contributes to sperm capacitation and acrosomal function during fertilization. Workplace Institute of Biotechnology Contact Monika Kopřivová, Monika.Koprivova@ibt.cas.cz, Tel.: 325 873 700 Year of Publishing 2013
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