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The role of auxins in somatic embryogenesis of Abies alba
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SYSNO ASEP 0367372 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title The role of auxins in somatic embryogenesis of Abies alba Author(s) Vondráková, Zuzana (UEB-Q) RID, ORCID
Eliášová, Kateřina (UEB-Q) RID, ORCID
Fischerová, Lucie (UEB-Q) RID, ORCID
Vágner, Martin (UEB-Q) RID, ORCIDSource Title Central European Journal of Biology - ISSN 1895-104X
Roč. 6, č. 4 (2011), s. 587-596Number of pages 10 s. Language eng - English Country PL - Poland Keywords Auxin inhibitor ; Fir ; Phytohormone Subject RIV EF - Botanics R&D Projects OC 158 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) CEZ AV0Z50380511 - UEB-Q (2005-2011) UT WOS 000290803200013 DOI 10.2478/s11535-011-0035-7 Annotation We report the effects of the synthetic auxin 2,4-dichlorophenoxyacetic acid (2,4-D) and the auxin inhibitor p-chlorophenoxyisobutyric acid (PCIB) on the endogenous level of the auxin indole-3-acetic acid (IAA) and on the anatomical composition of early somatic embryos of Abies alba (European silver fir). The embryogenic suspensor mass (ESM) of Abies alba proliferated on a medium supplemented by 2,4-D as well as on an auxin-free medium. The endogenous level of IAA was significantly higher in the ESM cultivated on a medium supplemented by 2,4-D. The decrease in the endogenous level of IAA in the first week of maturation is one of the most important stimuli responsible for the subsequent development of embryos. Suppression of IAA synthesis by an auxin inhibitor did not stimulate the development of embryos. The maturation of somatic embryos from the globular to the cotyledonary stage occurs when the concentration of endogenous auxin in the ESM (including the embryos) increases. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2012
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