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Antiviral activity of tenofovir against Cauliflower mosaic virus and its metabolism in Brassica pekinensis plants

SYSNO ASEP	0367346
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Článek ve WOS
Title	Antiviral activity of tenofovir against Cauliflower mosaic virus and its metabolism in Brassica pekinensis plants
Author(s)	Špak, Josef (BC-A)_{RID, ORCID} Votruba, Ivan (UOCHB-X)_RID Pavingerová, Daniela (BC-A) Holý, Antonín (UOCHB-X) Špaková, Vlastimila (BC-A) Petrzik, Karel (BC-A)_{RID, ORCID}
Number of authors	6
Source Title	Antiviral Research. - : Elsevier - ISSN 0166-3542 Roč. 92, č. 2 (2011), s. 378-381
Number of pages	4 s.
Language	eng - English
Country	NL - Netherlands
Keywords	Caulimovirus ; dsDNA ; Brassica
Subject RIV	CE - Biochemistry
R&D Projects	GA522/09/0707 GA ČR - Czech Science Foundation (CSF)
CEZ	AV0Z50510513 - UMBR-M, BC-A (2005-2011)
	AV0Z40550506 - UOCHB-X (2005-2011)
UT WOS	000297427200030
DOI	10.1016/j.antiviral.2011.08.014
Annotation	The antiviral effect of the acyclic nucleoside phosphonate tenofovir (R)-PMPA on double-stranded DNA Cauliflower mosaic virus (CaMV) in Brassica pekinensis plants grown in vitro on liquid medium was evaluated. Double antibody sandwich ELISA and PCR were used for relative quantification of viral protein and detecting nucleic acid in plants. (R)-PMPA at concentrations of 25 and 50 mg/l significantly reduced CaMV titers in plants within 6–9 weeks to levels detectable neither by ELISA nor by PCR. Virus-free plants were obtained after 3-month cultivation of meristem tips on semisolid medium containing 50 mg/l (R)-PMPA and their regeneration to whole plants in the greenhouse. Studying the metabolism of (R)-PMPA in B. pekinensis revealed that mono- and diphosphate, structural analogs of NDP and/or NTP, are the only metabolites formed. The data indicate very low substrate activity of the enzymes toward (R)-PMPA as substrate.
Workplace	Biology Centre (since 2006)
Contact	Dana Hypšová, eje@eje.cz, Tel.: 387 775 214
Year of Publishing	2012

Number of the records: 1